人BMP-4 ELISA试剂盒

• ELISA

Supplier Data

ELISA - Human BMP-4 ELISA Kit (AB231930)

• sELISA

Unknown

Sandwich ELISA - Human BMP-4 ELISA Kit (AB231930)

Human BMP-4 Competitor standard curve comparison.

Standard curve comparison between Human BMP-4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

• sELISA

Supplier Data

Sandwich ELISA - Human BMP-4 ELISA Kit (AB231930)

Example of human BMP-4 standard curve in Sample Diluent NS.

The BMP-4 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Human BMP-4 ELISA Kit (AB231930)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMP-4 concentration in neat serum was determined to be 163.5 pg/mL with a range of 69.95 – 505.8 pg/mL.

• sELISA

Supplier Data

Sandwich ELISA - Human BMP-4 ELISA Kit (AB231930)

Interpolated concentrations of native BMP-4 in human cell and tissue extract.

Interpolated concentrations of native BMP-4 in human HT-29 cell extract based on a 150 μg/mL extract load, HepG2 cell extract based on a 300 μg/mL extract load, and colon tissue extract based on a 1,000 μg/mL extract load. The concentrations of BMP-4 were measured in duplicate and interpolated from the BMP-4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMP-4 concentration was determined to be 456.7 pg/mL in HT-29 cell extract, 88.13 pg/mL in HepG2 cell extract, and 22.20 pg/mL in colon tissue extract.

• sELISA

Supplier Data

Sandwich ELISA - Human BMP-4 ELISA Kit (AB231930)

Interpolated concentrations of native BMP-4 in human cell culture supernatant samples.

The concentrations of BMP-4 were measured in duplicates, interpolated from the BMP-4 standard curves and corrected for sample dilution. Undiluted samples are as follows : SW480 cell culture supernatant 100%, HT-29 cell culture supernatant 50%, HepG2 cell culture supernatant 100%, and naïve culture media (100% RPMI + 10% FBS) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMP-4 concentration was determined to be 535.2 pg/mL in neat SW480 cell culture supernatant, 1035 pg/mL in neat HT-29 cell culture supernatant, 103.3 pg/mL in HepG2 cell culture supernatant, and 24.02 pg/mL in naïve culture media containing 10% FBS.

• sELISA

Supplier Data

Sandwich ELISA - Human BMP-4 ELISA Kit (AB231930)

Example of human BMP-4 standard curve in 1X Cell Extraction Buffer PTR.

The BMP-4 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Human BMP-4 ELISA Kit (AB231930)

Interpolated concentrations of native BMP-4 in human serum and plasma samples.

The concentrations of BMP-4 were measured in duplicates, interpolated from the BMP-4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMP-4 concentration was determined to be 193.9 pg/mL in neat serum, 162.0 pg/mL in neat plasma (citrate), and 91.1 pg/mL in neat plasma (EDTA).