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AB309280

人BFL-1/GRS ELISA试剂盒

Human BFL-1/GRS ELISA Kit

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Human BFL-1/GRS ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BFL-1/GRS with a sensitivity of 0.049 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

BCL2L5, BFL1, GRS, HBPA1, BCL2A1, Bcl-2-related protein A1, Bcl-2-like protein 5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS, Bcl2-L-5

2 Images
Sandwich ELISA - Human BFL-1/GRS ELISA Kit (AB309280)
  • sELISA

Supplier Data

Sandwich ELISA - Human BFL-1/GRS ELISA Kit (AB309280)

Interpolated concentrations of human BFL-1/GRS in U-937 cell extract

Interpolated concentration of native BFL-1/GRS was measured in duplicate at different sample concentrations. The undiluted sample is 250 µg/mL U-937 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human BFL-1/GRS ELISA Kit (AB309280)
  • sELISA

Supplier Data

Sandwich ELISA - Human BFL-1/GRS ELISA Kit (AB309280)

Example of human BFL-1/GRS standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell Lysate

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 0.049 ng/mL

范围

0.094 - 6 ng/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

Human BFL-1/GRS SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BFL-1/GRS protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human BFL-1/GRS with 0.049 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

精确度

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

回收率

[ { "sample": "Cell Lysate", "range": "90 - 95 %", "average": "= 92" } ]

规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C

产品实验方案

靶点信息

Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection (By similarity). Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11 (By similarity).
See full target information BCL2A1
websiteProtocolBooklet
en

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