AB99986

人Beta Nerve Growth Factor ELISA试剂盒(NGFB)

Name: 人Beta Nerve Growth Factor ELISA试剂盒 (NGFB) (ab99986)| Abcam中文官网
Brand: Abcam Limited
SKU: AB99986
Price: 7672 CNY
Availability: InStock
Rating: 4 (6 reviews)

Human Beta Nerve Growth Factor ELISA Kit (NGFB)

(6 Reviews)

(13 Publications)

Human Beta Nerve Growth Factor ELISA Kit (NGFB) is a Sandwich (quantitative) ELISA for the measurement of Human Beta Nerve Growth Factor (NGFB) in Human in Biofluids, Cell Culture Media samples.

查看别名

NGFB, NGF, Beta-nerve growth factor, Beta-NGF

1 Images

Sandwich ELISA - Human Beta Nerve Growth Factor ELISA Kit (NGFB) (AB99986)

sELISA

Supplier Data

Sandwich ELISA - Human Beta Nerve Growth Factor ELISA Kit (NGFB) (AB99986)

Representative Standard Curve using ab99986.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

< 14 pg/mL

范围

6.86 - 5000 pg/mL

检测平台

Microplate

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

产品详情

Abcam's Beta Nerve Growth Factor (NGFB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Beta Nerve Growth Factor in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human Beta Nerve Growth Factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Beta Nerve Growth Factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Beta Nerve Growth Factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Beta Nerve Growth Factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human beta Nerve Growth Factor ELISA Kit (ab193760) from our SimpleStep ELISA^® range.

NGFB concentration is very low in normal human serum samples and may be undetectable even with undiluted samples. Optimization may be required with urine samples.

style

left-column

回收率

[ { "sample": "Cell culture supernatant", "range": "90 - 122 %", "average": "= 106.7" }, { "sample": "Serum", "range": "98 - 116 %", "average": "= 113.1" }, { "sample": "Plasma", "range": "85 - 117 %", "average": "= 102.8" } ]

style

left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99986-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB99986-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99986-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99986-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Beta Nerve Growth Factor Microplate (12 x 8 wells)", "number":"AB99986-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99986-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Beta Nerve Growth Factor Standard Human (recombinant) (lyophilized)", "number":"AB99986-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Beta Nerve Growth Factor", "number":"AB99986-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB99986-CMP02", "productcode":"" } ] } } }

style

left-column

性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Beta nerve growth factor (beta-NGF) is a significant protein that functions in the development and maintenance of the nervous system. It is also known as NGF or NGFB and has a molecular mass of around 26 kDa. This protein is highly expressed in a wide range of tissues including the brain skin and various glands. Researchers often use assays like growth factor assays and NGF ELISA kits to measure beta-NGF levels given its importance in several physiological processes.

Biological function summary

Beta nerve growth factor plays an important role in the growth survival and differentiation of neurons. As a member of the neurotrophin family it binds to receptors like TrkA and p75NTR on the surface of nerve cells. This binding activity helps to promote neuronal health and function especially in the peripheral and central nervous systems. While beta-NGF traditionally acts independently it also interacts with other signaling molecules to trigger cascades that support cell survival and neural connections.

Pathways

Beta-NGF prominently participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for transmitting signals from the cell surface to the DNA in the nucleus therefore regulating gene expression linked to cell growth and survival. In these contexts beta-NGF works closely with proteins like Akt and ERK enhancing neuron resilience and promoting restorative mechanisms across the nervous system.

Beta nerve growth factor has been connected to neurodegenerative diseases like Alzheimer's disease and chronic pain conditions. Alterations in beta-NGF expression or signaling can impact neuronal survival contributing to the progression of these disorders. In Alzheimer's disease for example changes in NGF interactions with proteins like amyloid-beta and tau may exacerbate neurodegeneration. Similarly in pain syndromes disruptions in beta-NGF signaling can influence pain perception through pathways involving other neurotrophic factors.

style

left-column

产品实验方案

style

left-column

靶点信息

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed : 14976160, PubMed : 20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed : 20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed : 20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).

See full target information NGF

style

left-column

文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 48:1698-1713 PubMed39080995

2024

Overexpression of EGFL7 promotes angiogenesis and nerve regeneration in peripheral nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zengtao Hao,Zhiqi Huo,Qicheng Aixin-Jueluo,Tao Wu,Yihong Chen

Frontiers in immunology 13:783076 PubMed35300334

2022

Localization of Nerve Growth Factor Expression to Structurally Damaged Cartilaginous Tissues in Human Lumbar Facet Joint Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias F Seidel,Cordula Netzer,Véronique Chobaz,Thomas Hügle,Jeroen Geurts

Cell transplantation 30:9636897211035409 PubMed34318707

2021

Stamina-Enhancing Effects of Human Adipose-Derived Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Jung Yoon,Hye Rim Seong,Jangbeen Kyung,Dajeong Kim,Sangryong Park,Ehn-Kyoung Choi,Yun-Bae Kim,Dongsun Park

Cancer reports (Hoboken, N.J.) 4:e1300 PubMed33026185

2020

Localization and hypersecretion of nerve growth factor in breast phyllodes tumors: Evidence from a preliminary study.

Applications

Unspecified application

Species

Unspecified reactive species

Ashutosh Kumar,Khursheed Raza,Tapas C Nag,Anurag Srivastava,Ritu Sehgal

Spanish journal of psychiatry and mental health 16:137-142 PubMed32674992

2020

Acute effects of a session of electroconvulsive therapy on brain-derived neurotrophic factor plasma levels.

Applications

Unspecified application

Species

Unspecified reactive species

Miquel Bioque,Karina S Mac-Dowell,Cristina Font,Ana Meseguer,Elisabet Macau,Marta Garcia-Orellana,Marc Valentí,Juan C Leza,Miquel Bernardo

Stem cells (Dayton, Ohio) 36:844-855 PubMed29476565

2018

Human Periodontal Ligament-Derived Stem Cells Promote Retinal Ganglion Cell Survival and Axon Regeneration After Optic Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Ping Cen,Tsz Kin Ng,Jia-Jian Liang,Xi Zhuang,Xiaowu Yao,Gary Hin-Fai Yam,Haoyu Chen,Herman S Cheung,Mingzhi Zhang,Chi Pui Pang

Molecular oncology 11:1189-1207 PubMed28557340

2017

TrkA is a binding partner of NPM-ALK that promotes the survival of ALK T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Shi,Suraj Konnath George,Bhawana George,Choladda V Curry,Albina Murzabdillaeva,Serhan Alkan,Hesham M Amin

Scientific reports 6:37360 PubMed27853274

2016

Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target.

Applications

ELISA

Species

Human

Hamish T J Gilbert,Nathan Hodson,Pauline Baird,Stephen M Richardson,Judith A Hoyland

Reproductive biology and endocrinology : RB&E 14:29 PubMed27233989

2016

Effect of nerve growth factor on sperm quality in asthenozoosprmic men during cryopreservation.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Saeednia,Maryam Shabani Nashtaei,Hossein Bahadoran,Ashraf Aleyasin,Fardin Amidi

Scientific reports 5:16953 PubMed26586020

2015

Adipose-derived Stem Cell Conditioned Media Extends Survival time of a mouse model of Amyotrophic Lateral Sclerosis.

Applications

ELISA

Species

Unspecified reactive species

Christine V Fontanilla,Huiying Gu,Qingpeng Liu,Timothy Z Zhu,Changwei Zhou,Brian H Johnstone,Keith L March,Robert M Pascuzzi,Martin R Farlow,Yansheng Du

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

人Beta Nerve Growth Factor ELISA试剂盒(NGFB)

Human Beta Nerve Growth Factor ELISA Kit (NGFB)

Sandwich ELISA - Human Beta Nerve Growth Factor ELISA Kit (NGFB) (AB99986)