人beta Nerve Growth Factor ELISA试剂盒

产品详情

Human beta Nerve Growth Factor ELISA Kit ab193760 is a rapid single-wash 90-min Sandwich ELISA to measure Human beta Nerve Growth Factor in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 6 pg/mL.

How the assay works

Human beta Nerve Growth Factor SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human beta Nerve Growth Factor ELISA Kit ab193760 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Beta Nerve Growth Factor is involved in the development of maintenance of the sympathetic and sensory nervous systems. As a ligand for NTRK1 and NGFR receptors, it's involved in cell signaling cascades that regulate neuronal proliferation, differentiation, and survival.

Beta Nerve Growth Factor is also involved in modulation of the immune system and in the trauma response, and has been implicated in a variety of responses including memory and mental health.