人BCMA ELISA试剂盒
Human BCMA ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
Be the first to review this product! Submit a review
|
(2 Publications)
Human BCMA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BCMA with a sensitivity of 10.81 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
CD269, BCM, BCMA, TNFRSF17, Tumor necrosis factor receptor superfamily member 17, B-cell maturation protein
- ELISA
Supplier Data
ELISA - Human BCMA ELISA Kit (AB263875)
- sELISA
Supplier Data
Sandwich ELISA - Human BCMA ELISA Kit (AB263875)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 13.58 ng/mL with a range of 9.70 – 18.54 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human BCMA ELISA Kit (AB263875)
Interpolated concentrations of native BCMA in human cell culture supernatant and pooled-sex urine samples.
The concentrations of BCMA were measured in duplicates, interpolated from the BCMA standard curves and corrected for sample dilution. Undiluted samples are as follows : U266B1 supernatant 1 : 200 and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 142.48 ng/mL in U266B1 supernatant and 3.41 ng/mL in pooled-sex urine.
- sELISA
Supplier Data
Sandwich ELISA - Human BCMA ELISA Kit (AB263875)
Interpolated concentrations of native BCMA in human serum and plasma samples.
The concentrations of BCMA were measured in duplicates, interpolated from the BCMA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 2.5%, plasma (heparin) 2.5%, and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 25.96 ng/mL in serum, 17.77 ng/mL in plasma (citrate), 18.02 ng/ml in plasma (heparin), and 17.02 ng/mL in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human BCMA ELISA Kit (AB263875)
Example of human BCMA standard curve in Sample Diluent NS.
The BCMA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
反应性数据
产品详情
Human BCMA ELISA Kit ab263875 is a rapid single-wash 90-min Sandwich ELISA to measure Human BCMA in cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 10.81 pg/mL.
How the assay works
Human BCMA SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human BCMA ELISA Kit ab263875 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-BCMA antibody [EPR22457-30] - BSA and Azide free (Capture) ab267460
- Anti-BCMA antibody [EPR22457-28] - BSA and Azide free (Detector) ab267460
Tumor necrosis factor receptor superfamily member 17 (BCMA) is a receptor involved in B cell survival. BCMA activates the NF-kappa-B and JNK pathways. Aberrant expression of BCMA is observed on malignant B cells, including during multiple myeloma, Hodgkin's lymphoma, and Waldenstrom's macroglobulinemia.
精确度
回收率
规格
性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
靶点信息
文献 (2)
Recent publications for all applications. Explore the full list and refine your search
Haematologica 109:3670-3680 PubMed38572568
2024
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in immunology 12:647873 PubMed33828557
2021
Applications
Unspecified application
Species
Unspecified reactive species
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com