AB277466

人ARSB ELISA试剂盒

Name: 人ARSB ELISA试剂盒 (ab277466)| Abcam中文官网
Brand: Abcam Limited
SKU: AB277466
Price: 6412 CNY
Availability: InStock

Human ARSB ELISA Kit

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Human ARSB ELISA Kit is a Sandwich ELISA for the measurement of Human ARSB in Human in Biofluids, Cell Culture Media samples.

查看别名

Arylsulfatase B, ASB, N-acetylgalactosamine-4-sulfatase, G4S, ARSB

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ELISA

Supplier Data

ELISA - Human ARSB ELISA Kit (AB277466)

These standard curves are for demonstration only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

灵敏度

= 0.32 ng/mL

范围

0.307 - 75 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human ARSB ELISA Kit (ab277466) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ARSB in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human ARSB coated on a 96-well plate. Standards and samples are pipetted into the wells and Human ARSB present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human ARSB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human ARSB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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回收率

[ { "sample": "Serum", "range": "128 - 148 %", "average": "= 140" }, { "sample": "Plasma", "range": "133 - 146 %", "average": "= 141.5" }, { "sample": "Cell culture media", "range": "136 - 147 %", "average": "= 144" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277466-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277466-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277466-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277466-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human ARSB coated Microplate", "number":"AB277466-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ARSB standard protein (Lyophilized)", "number":"AB277466-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human ARSB Antibody", "number":"AB277466-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB277466-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277466-CMP07", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The ARSB protein also known as arylsulfatase B or N-acetylgalactosamine-4-sulfatase is an enzyme weighing approximately 62 kDa. It plays a role in breaking down glycosaminoglycans in the lysosomes. ARSB is highly expressed in tissues such as the liver kidney and skin where its enzymatic activity is important for normal cellular metabolism.

Biological function summary

ARSB functions in the degradation of dermatan sulfate and chondroitin sulfate types of glycosaminoglycans. It works as a monomer and is not part of a larger protein complex. This enzyme targets specific sulfated molecules facilitating their breakdown and recycling within the lysosomes.

Pathways

ARSB is actively involved in the lysosomal degradation pathway one of the key cellular processes for waste removal and recycling of macromolecules. This pathway includes collaboration with other lysosomal enzymes such as iduronate-2-sulfatase to complete the breakdown of complex molecules. ARSB's function in coordination with these enzymes supports cellular maintenance and metabolic balance.

ARSB malfunction is directly linked to the lysosomal storage disorder Mucopolysaccharidosis VI (Maroteaux-Lamy syndrome) characterized by the accumulation of glycosaminoglycans. This deficiency can lead to several symptoms including skeletal abnormalities and organomegaly. ARSB has also shown relevance in some inflammatory disorders where alterations in glycosaminoglycan breakdown impact cellular communication and tissue homeostasis.

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产品实验方案

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靶点信息

Removes sulfate groups from chondroitin-4-sulfate (C4S) and regulates its degradation (PubMed : 19306108). Involved in the regulation of cell adhesion, cell migration and invasion in colonic epithelium (PubMed : 19306108). In the central nervous system, is a regulator of neurite outgrowth and neuronal plasticity, acting through the control of sulfate glycosaminoglycans and neurocan levels (By similarity).

See full target information ARSB

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websiteProtocolBooklet

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人ARSB ELISA试剂盒

Human ARSB ELISA Kit

ELISA - Human ARSB ELISA Kit (AB277466)