人APRIL ELISA试剂盒(ab119505)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.78 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人APRIL ELISA试剂盒
参阅全部 APRIL/TNFSF13 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 8.1% 批次间 样品 n Mean SD CV% Overall 7.1% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.4 ng/ml -
范围
0.78 ng/ml - 50 ng/ml -
回收率
76 %
特定样本回收率 样品类型 平均% 范围 Serum 76 % - % -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s APRIL Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human APRIL concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).
APRIL specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an APRIL biotinylated detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of APRIL captured on the plate.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-human APRIL polyclonal antibody 1 x 70µl Human APRIL Standard 2 vials Microplate coated with polyclonal antibody to Human APRIL (12 x 8 wells) 1 unit Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究领域
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功能
Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. -
组织特异性
Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The precursor is cleaved by furin. -
细胞定位
Secreted. - Information by UniProt
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别名
- A proliferation inducing ligand
- A proliferation-inducing ligand
- APRIL
see all -
数据库链接
- Entrez Gene: 8741 Human
- Omim: 604472 Human
- SwissProt: O75888 Human
- Unigene: 54673 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119505于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab119505.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab119505 被引用在 1 文献中.
- Ortiz-Aljaro P et al. Protein and functional isoform levels and genetic variants of the BAFF and APRIL pathway components in systemic lupus erythematosus. Sci Rep 12:11219 (2022). PubMed: 35780200