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AB119505

人APRIL ELISA试剂盒

Human APRIL ELISA Kit

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(1 Publication)

Human APRIL ELISA Kit is a Sandwich ELISA for the measurement of Human APRIL in Human in Biofluids, Cell Culture Media samples.

查看别名

CD256, APRIL, TALL2, ZTNF2, UNQ383/PRO715, TNFSF13, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, TNF- and APOL-related leukocyte expressed ligand 2, TNF-related death ligand 1, TALL-2, TRDL-1

1 Images
Sandwich ELISA - Human APRIL ELISA Kit (AB119505)
  • sELISA

Supplier Data

Sandwich ELISA - Human APRIL ELISA Kit (AB119505)

Representative Standard Curve using ab119505.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 0.4 ng/mL

范围

0.78 - 50 ng/mL

检测平台

Microplate

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搭配使用产品

Assay Kits

AB188391

Human BAFF ELISA Kit (TNFSF13B)

SimpleStep

Sandwich ELISA - Human BAFF ELISA Kit (TNFSF13B) (AB188391)

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Assay Kits

AB213839

Human BAFF-R ELISA Kit

Sandwich ELISA - Human BAFF-R ELISA Kit (AB213839)

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Assay Kits

AB263875

Human BCMA ELISA Kit

Recombinant|SimpleStep

ELISA - Human BCMA ELISA Kit (AB263875)

  • 0
  • 0
Assay Kits

AB288592

Human TACI ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human TACI ELISA Kit (AB288592)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Abcam's APRIL Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human APRIL concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).

APRIL specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an APRIL biotinylated detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of APRIL captured on the plate.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" } ]
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回收率

[ { "sample": "Serum", "range": null, "average": "= 76" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119505-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"20X Assay Buffer Concentrate", "number":"AB119505-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB119505-CMP09", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Films", "number":"AB119505-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution (1M Phosphoric acid)", "number":"AB119505-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB119505-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate coated with polyclonal antibody to Human APRIL (12 x 8 wells)", "number":"AB119505-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent", "number":"AB119505-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human APRIL Standard", "number":"AB119505-CMP05", "productcode":"" }, { "size":"1 x 70 µL", "name":"Biotin-Conjugate anti-human APRIL polyclonal antibody", "number":"AB119505-CMP04", "productcode":"" }, { "size":"1 x 150 µL", "name":"Streptavidin-HRP", "number":"AB119505-CMP08", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

APRIL also known as TNFSF13 is a member of the tumor necrosis factor (TNF) ligand superfamily. This protein has a mass of approximately 31 kDa and undergoes incorporation into different cellular processes. APRIL is mainly expressed in cells of the immune system such as T cells B cells and myeloid cells. The protein plays a critical role in modulating various biological activities including cell survival proliferation and apoptosis.
Biological function summary

APRIL functions as a cytokine involved in immune regulation. The molecule acts independently or as part of a ligand-receptor interaction binding specifically to receptors like BCMA and TACI. This interaction influences B-cell maturation and differentiation driving immune responses. APRIL helps maintain homeostasis within the immune system ensuring appropriate immune activation and preventing overactive immune responses.

Pathways

APRIL coordinates with several immune-related pathways notably the B-cell signaling pathway and the NF-kB signaling pathway. These pathways are essential in immune system modulation influencing how the body responds to pathogens. APRIL interacts with proteins such as BAFF (B-cell activating factor) which share common receptors shaping the immune response through these pathways and ensuring proper immune cell function.

APRIL has connections with conditions like autoimmune diseases and certain lymphomas. Elevated levels of APRIL often occur in diseases like lupus contributing to abnormal B-cell activity and autoantibody production. Additionally its involvement in lymphomas links to excessive proliferation of B cells. Proteins such as BAFF and their receptors play a significant role in these conditions highlighting APRIL's impact on disease mechanisms and potential as a therapeutic target.
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产品实验方案

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靶点信息

Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
See full target information TNFSF13
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:11219 PubMed35780200

2022

Protein and functional isoform levels and genetic variants of the BAFF and APRIL pathway components in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Pilar Ortiz-Aljaro,Marco Antonio Montes-Cano,José-Raúl García-Lozano,Virginia Aquino,Rosario Carmona,Javier Perez-Florido,Francisco José García-Hernández,Joaquín Dopazo,María Francisca González-Escribano
View all publications
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websiteProtocolBooklet
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