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人Apolipoprotein H ELISA试剂盒(APOH) (ab108814)

Submit a review Q&A (1)
108814 - Sandwich elisa
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.13 ng/ml
  • Range: 0.313 ng/ml - 20 ng/ml
  • Sample type: Milk, Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

概述

  • 产品名称

    人Apolipoprotein H ELISA试剂盒(APOH)

  • 检测方法

    Colorimetric

  • 精确度

    批次内
    样品 n Mean SD CV%
    Overall 3.5%
    批次间
    样品 n Mean SD CV%
    Overall 9.7%

  • 样品类型

    Saliva, Milk, Urine, Serum, Plasma

  • 检测类型

    Sandwich (quantitative)

  • 灵敏度

    = 0.13 ng/ml

  • 范围

    0.313 ng/ml - 20 ng/ml

  • 回收率

    98 %

  • 检测时间

    4h 00m

  • 实验步骤

    Multiple steps standard assay

  • 种属反应性

    与反应: Human

  • 产品概述

    Abcam’s Apolipoprotein H (APOH) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein H concentrations in Human plasma, serum, saliva, urine, milk and cell culture samples.


    An Apolipoprotein H specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein H specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein H captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • 经测试应用

    适用于: Sandwich ELISAmore details

  • 平台

    Microplate

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab108814于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Sandwich ELISA
Use at an assay dependent concentration.
说明
Sandwich ELISA
Use at an assay dependent concentration.

图片

  • 108814 - Sandwich elisa
    108814 - Sandwich elisa

    Apolipoprotein H measured in biological fluids with background signal subtracted (duplicates +/- SD).The samples were tested in a range of dilutions (human serum and plasma at 1:10000-1:200000; milk at 1:100-1:1000 and the rest 1:1-1:50).

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108814

文献 (0)

发表研究结果有使用 ab108814？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab108814 尚未被引用在任何文献中。

客户评价及客户问答

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Question


I have a second question: unfortunately, there is no expiry date stated on the kits. Could you please help me out on that?

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Answer



Our guarantee period is 12 month from the date of purchase and therefore I suggest to also take this date as expiry date for un-opened kits that are stored as described in the protocol booklet.

When reagents are opened they also can be stored for a short vial (please refer to the protocol booklets) but they will expire very shortly after openeing.

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