人Apolipoprotein A-IV ELISA试剂盒
Human Apolipoprotein A-IV ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
4
(1 Review)
|
(4 Publications)
Human Apolipoprotein A-IV ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Apolipoprotein A-IV with a sensitivity of 5.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4, APOA4
- ELISA
Supplier Data
ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean human Apolipoprotein A-IV concentration was determined to be 192.8 μg/mL with a range of 9.2 – 418.0 μg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
Interpolated concentrations of native Apolipoprotein A-IV in human saliva and urine samples.
Saliva was analyzed at 6.25% and urine at 12.5%. The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 25,370 pg/mL in neat saliva (native) and 3903 pg/mL in neat urine (native).
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
Interpolated concentrations of native Apolipoprotein A-IV in human placenta and human liver homogenate tissue extracts based on 500 μg/mL and 400 µg/mL extract loads, respectively.
The concentrations of Apolipoprotein A-IV were measured in duplicate and interpolated from the Apolipoprotein A-IV standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 4,829 pg/mL in placenta extract and 17,016 pg/mL in liver homogenate extract.
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
Example of human Apolipoprotein A-IV standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
Interpolated concentrations of spiked Apolipoprotein A-IV in human saliva and urine samples.
The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 6.25% and urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 177,941 pg/mL in neat saliva (spiked) and 80,680 pg/mL in neat urine (spiked).
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
Interpolated concentrations of native Apolipoprotein A-IV in human serum and plasma samples.
The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 32,000, plasma (citrate) 1 : 16,000, plasma (EDTA) 1 : 16,000, and plasma (heparin) 1 : 16000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 71.65 μg/mL in neat serum, 48.04 μg/mL in neat plasma (citrate), 42.94 μg/mL in neat plasma (EDTA) and 51.18 μg/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
Example of human Apolipoprotein A-IV standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
反应性数据
产品详情
Human Apolipoprotein A-IV ELISA Kit ab214567 is a rapid single-wash 90-min Sandwich ELISA to measure Human Apolipoprotein A-IV in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum, tissue extracts, urine. This SimpleStep sensitivity is 5.4 pg/mL.
How the assay works
Human Apolipoprotein A-IV SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Apolipoprotein A-IV ELISA Kit ab214567 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-APOA4/Apo-AIV antibody [EPR19465-232] - BSA and Azide free (Capture) ab242896
- Anti-APOA4/Apo-AIV antibody [EPR19465-41] - BSA and Azide free (Detector) ab242896
Human Apolipoprotein A-IV is a 45kDa glycoprotein with activities involving lipid transport. Specifically, Apolipoprotein A-IV is a component of high density lipoprotein (HDL) and chylomicrons. Its primarily synthesized is the intestine in association with lymph chylomicron particles. Although its precise function is not known, Apolipoprotein A-IV is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. Human Apolipoprotein A-IV consists of a 20 amino acid (aa) signal peptide and 376 aa mature chain. Human Apolipoprotein A-IV is 62% identical to both mouse and rat Apolipoprotein A-IV.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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性能和储存信息
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推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Apolipoprotein A4 acts as an important player in the regulation of lipid homeostasis and satiety. This protein is not part of a complex but enhances the efficiency of lipid absorption and transport. APOA4 reacts with enzymes like lecithin-cholesterol acyltransferase which is essential in the maturation of HDL particles. This interaction supports cholesterol efflux and assists in maintaining cholesterol balance within the body.
Pathways
APOA4 is heavily involved in the reverse cholesterol transport pathway which is important for removing excess cholesterol from cells and tissues taking it back to the liver for excretion. Within this pathway APOA4 interacts with key proteins like apolipoprotein A1 and lecithin-cholesterol acyltransferase to aid in this lipid transport mechanism. It also participates in a related lipid homeostasis pathway enhancing lipid absorption efficiency.
靶点信息
文献 (4)
Recent publications for all applications. Explore the full list and refine your search
Nature medicine 31:2213-2221 PubMed40186079
2025
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Proceedings of the National Academy of Sciences of the United States of America 121:e2411189121 PubMed39475639
2024
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ACS chemical neuroscience 13:1030-1045 PubMed35200010
2022
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Molecular medicine reports 17:2121-2126 PubMed29207081
2017
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