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AB214567

人Apolipoprotein A-IV ELISA试剂盒

Human Apolipoprotein A-IV ELISA Kit

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(4 Publications)

Human Apolipoprotein A-IV ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Apolipoprotein A-IV with a sensitivity of 5.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4, APOA4

8 Images
ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
  • ELISA

Supplier Data

ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean human Apolipoprotein A-IV concentration was determined to be 192.8 μg/mL with a range of 9.2 – 418.0 μg/mL.

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)

Interpolated concentrations of native Apolipoprotein A-IV in human saliva and urine samples.

Saliva was analyzed at 6.25% and urine at 12.5%. The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 25,370 pg/mL in neat saliva (native) and 3903 pg/mL in neat urine (native).

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)

Interpolated concentrations of native Apolipoprotein A-IV in human placenta and human liver homogenate tissue extracts based on 500 μg/mL and 400 µg/mL extract loads, respectively.

The concentrations of Apolipoprotein A-IV were measured in duplicate and interpolated from the Apolipoprotein A-IV standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 4,829 pg/mL in placenta extract and 17,016 pg/mL in liver homogenate extract.

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)

Example of human Apolipoprotein A-IV standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)

Interpolated concentrations of spiked Apolipoprotein A-IV in human saliva and urine samples.

The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 6.25% and urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 177,941 pg/mL in neat saliva (spiked) and 80,680 pg/mL in neat urine (spiked).

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)

Interpolated concentrations of native Apolipoprotein A-IV in human serum and plasma samples.

The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 32,000, plasma (citrate) 1 : 16,000, plasma (EDTA) 1 : 16,000, and plasma (heparin) 1 : 16000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 71.65 μg/mL in neat serum, 48.04 μg/mL in neat plasma (citrate), 42.94 μg/mL in neat plasma (EDTA) and 51.18 μg/mL in neat plasma (heparin).

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)

Example of human Apolipoprotein A-IV standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Saliva, Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 5.4 pg/mL

范围

250 - 16000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

Human Apolipoprotein A-IV ELISA Kit ab214567 is a rapid single-wash 90-min Sandwich ELISA to measure Human Apolipoprotein A-IV in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum, tissue extracts, urine. This SimpleStep sensitivity is 5.4 pg/mL.

How the assay works

Human Apolipoprotein A-IV SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Apolipoprotein A-IV ELISA Kit ab214567 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-APOA4/Apo-AIV antibody [EPR19465-232] - BSA and Azide free (Capture) ab242896
- Anti-APOA4/Apo-AIV antibody [EPR19465-41] - BSA and Azide free (Detector) ab242896

Human Apolipoprotein A-IV is a 45kDa glycoprotein with activities involving lipid transport. Specifically, Apolipoprotein A-IV is a component of high density lipoprotein (HDL) and chylomicrons. Its primarily synthesized is the intestine in association with lymph chylomicron particles. Although its precise function is not known, Apolipoprotein A-IV is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. Human Apolipoprotein A-IV consists of a 20 amino acid (aa) signal peptide and 376 aa mature chain. Human Apolipoprotein A-IV is 62% identical to both mouse and rat Apolipoprotein A-IV.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]

回收率

[ { "sample": "Serum", "range": "90 - 101 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "102 - 107 %", "average": "= 104" }, { "sample": "Urine", "range": "95 - 98 %", "average": "= 97" }, { "sample": "Saliva", "range": "76 - 79 %", "average": "= 78" }, { "sample": "Tissue Extracts", "range": "102 - 105 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "95 - 101 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "90 - 94 %", "average": "= 91" }, { "sample": "Cell culture media", "range": "102 - 103 %", "average": "= 103" } ]

规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

APOA4 also known as Apo-AIV or apolipoprotein A4 is a protein mainly expressed in the small intestine and liver. It has a molecular mass of approximately 46 kDa. This protein plays a significant role in lipid metabolism by facilitating the transport of lipids in the bloodstream. APOA4 exists in the plasma and associates predominately with high-density lipoproteins (HDL) and to a lesser extent with chylomicrons.
Biological function summary

Apolipoprotein A4 acts as an important player in the regulation of lipid homeostasis and satiety. This protein is not part of a complex but enhances the efficiency of lipid absorption and transport. APOA4 reacts with enzymes like lecithin-cholesterol acyltransferase which is essential in the maturation of HDL particles. This interaction supports cholesterol efflux and assists in maintaining cholesterol balance within the body.

Pathways

APOA4 is heavily involved in the reverse cholesterol transport pathway which is important for removing excess cholesterol from cells and tissues taking it back to the liver for excretion. Within this pathway APOA4 interacts with key proteins like apolipoprotein A1 and lecithin-cholesterol acyltransferase to aid in this lipid transport mechanism. It also participates in a related lipid homeostasis pathway enhancing lipid absorption efficiency.

Research links APOA4 to atherosclerosis and obesity. Altered expression or function of apolipoprotein A4 often correlates with increased cardiovascular disease risk due to its role in cholesterol transport. It is associated with apolipoprotein A1 as both of these proteins significantly impact lipid profiles related to cardiovascular health. The ability of APOA4 to influence satiety also connects it to obesity suggesting a role in energy balance regulation.

产品实验方案

靶点信息

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
See full target information APOA4

文献 (4)

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