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Cardiovascular Blood Coagulation Intrinsic
重组SimpleStep

人Apo-H ELISA试剂盒(Apolipoprotein H) (ab274403)

  • Datasheet
  • SDS
  • Protocol Booklet
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SimpleStep ELISA Protocol Diagram
  • Example of human Apo-H standard curve in Sample Diluent NS.
  • Interpolated concentrations of native Apo-H in human serum and plasma samples.
  • Interpolated concentrations of native Apo-H in human HepG2 Cell extract based on a 200 µg/mL extract load.
  • Sandwich ELISA - Human Apo-H ELISA Kit (Apolipoprotein H) (ab274403)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 10.4 pg/ml
  • Range: 23.44 pg/ml - 1500 pg/ml
  • Sample type: Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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概述

  • 产品名称

    人Apo-H ELISA试剂盒(Apolipoprotein H)
    参阅全部 Apo-H 试剂盒
  • 检测方法

    Colorimetric
  • 精确度

    批次内
    样品 n Mean SD CV%
    Serum 8 1.7%
    批次间
    样品 n Mean SD CV%
    Serum 3 2.99%
  • 样品类型

    Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma
  • 检测类型

    Sandwich (quantitative)
  • 灵敏度

    10.4 pg/ml
  • 范围

    23.44 pg/ml - 1500 pg/ml
  • 回收率

    特定样本回收率
    样品类型 平均% 范围
    Serum 100 95% - 104%
    Cell Lysate 93 88% - 101%
    Hep Plasma 103 94% - 118%
    EDTA Plasma 101 96% - 107%
    Cit plasma 99 96% - 107%
  • 检测时间

    1h 30m
  • 实验步骤

    One step assay
  • 种属反应性

    与反应: Human
  • 产品概述

    Human Apo-H ELISA kit (ab274403) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Apo-H protein in serum, plasma and lystates. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Apo-H with 10.4 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 说明

    Apo-H, also known as beta-2 glycoprotein 1, is a key serum protein involved in agglutination response in the blood. It contains a highly positively charged stretch of amino acids which bind negatively charged agglutinizing factors. Improper expression of apo-H has been noted in many vascular disease states such as, cardiovascular disease, diabetes, and thrombosis. The immunogen used to raise the antibodies for this kit was the full-length mature protein. This protein does not share high sequence identity in mouse or rat samples (76.1 and 82.2%, respectively) and, therefore, is not expected to cross-react.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • 经测试应用

    适用于: Sandwich ELISAmore details
  • 平台

    Pre-coated microplate (12 x 8 well strips)

性能

  • 存放说明

    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    10X Human Apo-H (Apolipoprotein H) Capture Antibody 1 x 600µl
    10X Human Apo-H (Apolipoprotein H) Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human Apo-H (Apolipoprotein H) Lyophilized Recombinant Protein 2 vials
    Plate Seal 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究领域

    • Cardiovascular
    • Blood
    • Coagulation
    • Intrinsic
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Other
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
  • 功能

    Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
  • 组织特异性

    Expressed by the liver and secreted in plasma.
  • 序列相似性

    Contains 4 Sushi (CCP/SCR) domains.
  • 翻译后修饰

    N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele.
  • 细胞定位

    Secreted.
  • Target information above from: UniProt accession P02749 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 别名

    • Activated protein C binding protein
    • Activated protein C-binding protein
    • Anticardiolipin cofactor
    • APC inhibitor
    • Apo-H
    • APOH
    • APOH_HUMAN
    • Apolipoprotein H
    • apolipoprotein H (beta-2-glycoprotein I)
    • B2G1
    • B2GP1
    • B2GPI
    • Beta 2 glycoprotein I
    • Beta 2 glycoprotein I precursor
    • Beta(2)GPI
    • Beta-2-glycoprotein 1
    • Beta-2-glycoprotein I
    • BG
    • Glycoprotein 1, beta-2
    • Glycoprotein I, beta-2
    • OTTMUSP00000003033
    see all
  • 数据库链接

    • Entrez Gene: 350 Human
    • Omim: 138700 Human
    • SwissProt: P02749 Human
    • Unigene: 445358 Human

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab274403于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Sandwich ELISA
    Use at an assay dependent concentration.
    说明
    Sandwich ELISA
    Use at an assay dependent concentration.

    图片

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of human Apo-H standard curve in Sample Diluent NS.
      Example of human Apo-H standard curve in Sample Diluent NS.

      The Apo-H standard curve was prepared as described in Section 10.1. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native Apo-H in human serum and plasma samples.
      Interpolated concentrations of native Apo-H in human serum and plasma samples.

      The concentrations of Apo-H were measured in duplicates, interpolated from the Apo-H standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:140,000, plasma (Heparin) 1:80,000, plasma (EDTA) 1:80,000 and plasma (Citrate) 1:80,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-H concentration was determined to be 164.87 µg/ml in serum, 91.63 µg/ml in plasma (Heparin) and 82.17 µg/ml in plasma (EDTA) and 82.25 µg/ml in plasma (Citrate).

    • Interpolated concentrations of native Apo-H in human HepG2 Cell extract based on a 200 µg/mL extract load.
      Interpolated concentrations of native Apo-H in human HepG2 Cell extract based on a 200 µg/mL extract load.

      The concentrations of Apo-H were measured in duplicate and interpolated from the Apo-H standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-H concentration was determined to be 1307.93 pg/mL.

    • Sandwich ELISA - Human Apo-H ELISA Kit (Apolipoprotein H) (ab274403)
      Sandwich ELISA - Human Apo-H ELISA Kit (Apolipoprotein H) (ab274403)
      To learn more about the advantages of recombinant antibodies see here.

    实验方案

    • Protocol Booklet

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

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