AB267808

人Annexin A1 ELISA试剂盒

Human Annexin A1 ELISA Kit

Recombinant
SimpleStep
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Human Annexin A1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Annexin A1 with a sensitivity of 0.16 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

ANX1, LPC1, ANXA1, Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35

2 Images

Sandwich ELISA - Human Annexin A1 ELISA Kit (AB267808)

sELISA

Supplier Data

Sandwich ELISA - Human Annexin A1 ELISA Kit (AB267808)

Interpolated concentrations of native Annexin A1 in human A549 cell extract based on a 1.25 μg/mL extract load.

The concentrations of Annexin A1 were measured in duplicate and interpolated from the Annexin A1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Annexin A1 ELISA Kit (AB267808)

Example of human Annexin A1 (ANXA1) standard curve in 1X Cell Extraction Buffer PTR.

The Annexin A1 (ANXA1) standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell Lysate

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 0.16 ng/mL

范围

0.3125 - 20 ng/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human Annexin A1 ELISA Kit (ab267808) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Annexin A1 protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Annexin A1 with 0.16 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Annexin A1, also known as lipocortin I, annexin I, chromobindin-9, and calpactin II, belongs to the annexin family of calcium-dependent phospholipid-binding proteins. It mimics the effect of steroids, mediates anti-inflammatory activity and is a potent inhibitor of phospholipase A2 activity. Annexin A1 regulates neutrophil extravasation by interacting with the formyl peptide receptor and triggers different signaling pathways.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]

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回收率

[ { "sample": "Cell Lysate", "range": "108 - 116 %", "average": "= 111" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267808-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB267808-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB267808-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Annexin A1 Lyophilized Recombinant Protein", "number":"AB267808-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB267808-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB267808-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Annexin A1 Capture Antibody", "number":"AB267808-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB267808-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB267808-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Annexin A1 Detector Antibody", "number":"AB267808-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB267808-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB267808-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB267808-CMP08", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Annexin A1 also known as ANXA1 or annexin 1 is a multifunctional protein with a molecular mass of approximately 37 kDa. This protein belongs to the annexin family and is distributed widely in various tissue types including the central nervous system respiratory system and immune cells. Annexin A1 functions by binding to phospholipids in a calcium-dependent manner influencing membrane-related processes and signal transduction. Its expression can be examined using techniques like annexin A1 immunohistochemistry and annexin A1 ELISA to study protein localization and concentration.

Biological function summary

Annexin A1 is involved in modulating inflammatory responses and cell proliferation. It acts as a mediator in the resolution of inflammation by promoting apoptotic cell clearance and regulating leukocyte migration. Annexin A1 participates in complex interactions often associating with cell membrane phospholipids to exert its effects. It contributes significantly to cell adhesion migration and intracellular signaling events which are important for maintaining homeostasis and proper immune function.

Pathways

Annexin A1 plays a significant role in the glucocorticoid signaling pathway and the formation of the cytoskeleton. It interacts with proteins like lipocortin 1 and phospholipase A2 impacting processes such as arachidonic acid metabolism and cytoskeletal reorganization. These interactions underline annexin A1's involvement in regulating inflammation and maintaining cellular architecture linking it to cellular processes that govern stress responses and cell movement.

Annexin A1 exhibits relevance to inflammatory diseases and cancer. It has been implicated in conditions such as asthma where it modulates inflammatory cell recruitment and activation. In cancer especially in breast cancer annexin A1 influences tumor progression by interacting with proteins like COX-2 affecting tumor cell invasion and metastasis. The protein's role in these conditions highlights its potential as both a biomarker and therapeutic target helping in understanding disease mechanisms and developing treatment strategies.

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产品实验方案

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靶点信息

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed : 8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed : 17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed : 17008549). Has no effect on unstimulated T cells (PubMed : 17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed : 19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed : 2532504, PubMed : 8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).. Annexin Ac2-26. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed : 15187149, PubMed : 22879591, PubMed : 25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed : 15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed : 15187149). Promotes resolution of inflammation and wound healing (PubMed : 25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed : 22879591).

See full target information ANXA1

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websiteProtocolBooklet

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人Annexin A1 ELISA试剂盒

Human Annexin A1 ELISA Kit

Sandwich ELISA - Human Annexin A1 ELISA Kit (AB267808)

Sandwich ELISA - Human Annexin A1 ELISA Kit (AB267808)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台