人Amphiregulin ELISA试剂盒

产品详情

Human Amphiregulin ELISA Kit (ab222504) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Amphiregulin protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Amphiregulin with 2.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Amphiregulin is a member of the epidermal growth factor family encoded by the AREG gene. Amphiregulin is expressed as a 252- amino acid transmembrane glycoprotein, which contains an 81-amino acid N-terminal pro-peptide, a heparin binding domain, a EGF like domain, a 23- amino acid transmembrane domain, and a 31- amino acid cytoplasmic domain. The amphiregulin assay utilizes antibodies raised against the 101-187 amino acid region of the amphiregulin protein. Amphiregulin is an autocrine growth factor, a mitogen for several cell types, and is essential for mammary ductal development.