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AB240678

人alpha SMA ELISA试剂盒

Human alpha SMA ELISA Kit

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(9 Publications)

Human alpha SMA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human alpha SMA with a sensitivity of 0.2 ng/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

查看别名

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

6 Images
ELISA - Human alpha SMA ELISA Kit (AB240678)
  • ELISA

Supplier Data

ELISA - Human alpha SMA ELISA Kit (AB240678)
Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)
  • sELISA

Lab

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Interpolated concentration of native Human alpha SMA was measured in duplicate at different sample concentrations. Undiluted samples are 0.5 µg/mL human colon tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)
  • sELISA

Lab

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Example of species Human alpha SMA standard curve in 96-well vs. 384-well plate in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)
  • sELISA

Lab

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Interpolated concentration of native Human alpha SMA was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 0.5 µg/mL human colon tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)
  • sELISA

Lab

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Example of species Human alpha SMA standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Representative image of Human alpha SMA ELISA Kit (ab240678)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human alpha SMA Capture Antibody

- 10X Human alpha SMA Detector Antibody

- Human alpha SMA Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

关键信息

检测方法

Colorimetric

样品类型

Tissue Extracts

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 0.2 ng/mL

范围

0.7 - 45 ng/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

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Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

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Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

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Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

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Assay Kits

AB219046

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  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human alpha SMA ELISA Kit ab240678 is a rapid single-wash 90-min Sandwich ELISA to measure Human alpha SMA in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 21.5 pg/mL.

How the assay works

Human alpha SMA SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human alpha SMA ELISA Kit ab240678 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human alpha SMA ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-alpha smooth muscle Actin antibody [EPR20062-47] - BSA and Azide free (Capture) ab244818
- Anti-alpha smooth muscle Actin antibody [EPR20062-77] - BSA and Azide free (Detector) ab245091

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Colon", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Colon", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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回收率

[ { "sample": "Tissue Extracts", "range": "89 - 112 %", "average": "= 101" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (?-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of alpha-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of alpha-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally alpha-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of alpha-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of alpha-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of alpha-SMA in disease development.
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产品实验方案

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靶点信息

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
See full target information ACTA2
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