人alpha Fetoprotein ELISA试剂盒(AFP) (ab108838)
Key features and details
- Sensitivity: 0.38 ng/ml
- Range: 0.625 ng/ml - 40 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人alpha Fetoprotein ELISA试剂盒(AFP)
参阅全部 alpha 1 Fetoprotein 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4.1% 批次间 样品 n Mean SD CV% Overall 10.5% -
样品类型
Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.38 ng/ml -
范围
0.625 ng/ml - 40 ng/ml -
回收率
104 %
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检测时间
4h 0m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s alpha Fetoprotein (AFP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Fetoprotein levels in plasma, serum, cell lysate and culture supernatants, and tissue samples.
An alpha Fetoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Fetoprotein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Fetoprotein captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human alpha Fetoprotein Antibody 1 x 120µl alpha Fetoprotein Microplate (12 x 8 well strips) 1 unit alpha Fetoprotein Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
组织特异性
Plasma. Synthesized by the fetal liver and yolk sac. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
发展阶段
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻译后修饰
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
细胞定位
Secreted. - Information by UniProt
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别名
- Afp
- AFPD
- Alpha fetoglobulin
see all -
数据库链接
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108838于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108838.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab108838 被引用在 11 文献中.
- Zabady S et al. Cyanidin-3-Glucoside Modulates hsa_circ_0001345/miRNA106b/ATG16L1 Axis Expression as a Potential Protective Mechanism against Hepatocellular Carcinoma. Curr Issues Mol Biol 44:1677-1687 (2022). PubMed: 35723373
- Carbonaro M et al. Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model. Sci Rep 12:14079 (2022). PubMed: 35982097
- Aboughaleb IH et al. Integration of transcriptomes analysis with spectral signature of total RNA for generation of affordable remote sensing of Hepatocellular carcinoma in serum clinical specimens. Heliyon 7:e06388 (2021). PubMed: 33748469
- Kats D et al. Volasertib preclinical activity in high-risk hepatoblastoma. Oncotarget 10:6403-6417 (2019). PubMed: 31741706
- Lv S et al. H2-Based Electrochemical Biosensor with Pd Nanowires@ZIF-67 Molecular Sieve Bilayered Sensing Interface for Immunoassay. Anal Chem 91:12055-12062 (2019). PubMed: 31436433