人Alpha-1-Microglobulin ELISA试剂盒(ab226899)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 725 pg/ml
- Range: 1.56 ng/ml - 100 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Alpha-1-Microglobulin ELISA试剂盒
参阅全部 Alpha 1 microglobulin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 5 3.2% 批次间 样品 n Mean SD CV% Serum 3 5.8% -
样品类型
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
725 pg/ml -
范围
1.56 ng/ml - 100 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Urine 104 95% - 109% Serum 96 92% - 102% Cell culture media 106 103% - 109% Hep Plasma 92 88% - 96% EDTA Plasma 97 94% - 102% Cit plasma 96 95% - 97% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human
不与反应: Cow -
产品概述
Human Alpha-1-Microglobulin ELISA Kit (ab226899) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Alpha-1-Microglobulin protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Alpha-1-Microglobulin with 725 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Alpha 1-microglobulin is expressed in the liver and secreted in the plasma acting as a radical scavenger and degrades heme. Alpha 1-Microglobulin can exist as either a monomer or in complexes with IgA or Albumin. The precursor to Alpha 1-microglobulin (Protein AMBP) is cleaved into three separate functioning proteins (Alpha 1-microglobulin, Bikunin, and Trypstatin). Ab226899 is designed to recognize Alpha-1-microglobin, specificity over the pre-cursor has not been tested.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 96 tests 10X Human Alpha 1-Microglobulin Capture Antibody 1 x 600µl 1 x 600µl 10X Human Alpha 1-Microglobulin Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml Antibody Diluent CPR2 1 x 6ml 1 x 6ml Human Alpha 1-Microglobulin Lyophilized Recombinant Protein 2 vials 2 vials Plate Seal 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 unit Stop Solution 1 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 1 x 12ml -
研究领域
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功能
Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
Trypstatin is a trypsin inhibitor. -
组织特异性
Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine. -
序列相似性
In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
Contains 2 BPTI/Kunitz inhibitor domains. -
翻译后修饰
The precursor is proteolytically processed into separately functioning proteins.
3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components. -
细胞定位
Secreted. - Information by UniProt
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别名
- A1M
- Alpha 1 microglobulin/bikunin precursor
- Alpha 1 microglycoprotein
see all -
数据库链接
- Entrez Gene: 259 Human
- Omim: 176870 Human
- SwissProt: P02760 Human
- Unigene: 436911 Human
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226899于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Other - Human Alpha-1-Microglobulin ELISA Kit (ab226899)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of HumanAlpha 1-Microglobulin standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Alpha 1-Microglobulin in Human serum and plasma samplesThe concentrations of Alpha 1-Microglobulin were measured in duplicates, interpolated from the Alpha 1-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (EDTA) 1:100, plasma (heparin) 1:100, and plasma (citrate) 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha 1-Microglobulin concentration was determined to be 29.7 µg/mL in serum, 20.7 µg/mL in plasma (EDTA), 23.2 µg/mL in plasma (heparin) and 21.2 µg/mL in plasma (citrate).
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Serum from ten individual healthy Human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha 1-Microglobulin concentration was determined to be 27.4 µg/mL with a range of 12.6 – 41.9 µg/mL.
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Interpolated concentrations of native Alpha 1-Microglobulin in Human cell culture supernatant and urine samplesThe concentrations of Alpha 1-Microglobulin were measured in duplicates, interpolated from the Alpha 1-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: HEPG2 Cell culture supernatant 10% and urine 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha 1-Microglobulin concentration was determined to be 1026 ng/mL in HEPG2 cell culture supernatant and 9590 ng/mL in urine.
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Sandwich ELISA - Human Alpha-1-Microglobulin ELISA Kit (ab226899)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
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