人alpha 1 Antitrypsin ELISA试剂盒(SERPINA1) (ab108799)

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ab108798 is for plasma and serum samples.

ab108799 is for urine, milk, saliva and cell culture samples

Unfortunately, neither of them has cross-reactivity with mouse

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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You can use ab108799 for serum, you will just have to optimize the dilutions to get on the standard curve. Our serum samples were at 6000X.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your message.

I can confirm that all three of the Alpha 1 anti trypsin proteins in our catalog are from human, and are full length. Therefore, theoretically, they could be detected by the antibodies in the kit and be used for your experiments. However, please note they have not been tested in ELISA or with this kit, so regrettably we are not able to guarantee them for this purpose.

ab91136 alpha 1 Antitrypsin protein (Active)
Tested applications: SDS-PAGE, WB, FuncS
https://www.abcam.com/Native-human-alpha-1-Antitrypsin-protein-Active-ab91136.html (or use the following: https://www.abcam.com/Native-human-alpha-1-Antitrypsin-protein-Active-ab91136.html).

ab77937 alpha 1 Antitrypsin protein (Human)
Tested applications: SDS-PAGE
https://www.abcam.com/Native-Human-alpha-1-Antitrypsin-protein-ab77937.html (or use the following: https://www.abcam.com/Native-Human-alpha-1-Antitrypsin-protein-ab77937.html).

ab79181 alpha 1 Antitrypsin protein (Human)
Tested applications: SDS-PAGE
https://www.abcam.com/Recombinant-Human-alpha-1-Antitrypsin-protein-ab79181.html (or use the following: https://www.abcam.com/Recombinant-Human-alpha-1-Antitrypsin-protein-ab79181.html).

I am sorry we have no data to confirm these would work in this case. However, I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

Thank you for contacting Abcam and I do apologize for the long delay in providing you with this information.

The standard is a purified protein from human source. The assay should work for cell culture over express alpha1 antitripsin. Both native and recombinant protein should work in this assay unless the recombinant protein is a mutant.

If there is anything else I can help you with, please let me know.

Thank you for contacting us. The standard provided should only be 100 ng/ml after reconstitution. At that concentration, the recommended curve is a 1:4 dilution providing points: 25, 6.25, 1.56, and 0.391 ng/ml. Concentrations above 100 ng/ml are dangerous to calculate from as the antibody on the plate becomes saturated.

Running a 1:2 dilution of this standard, gives OD's that are very close together, and sometimes makes it difficult to produce a suitable curve. It may help to calculate your standard curve from the 25, 6.25, 1.56, and 0.391 ng/ml points in your data.

I hope this helps, please let me know if you need any additional information or assistance.

Thank you very much for your reply.

We haven't done any studies to determine the heterogeneity of the detection antibody reagent and I don't know of amethod to really predict that accurately as it's quite complex, but Iassumethere would be ballpark 10-50 different binding sites. I'm basing this mostly on the length of the protein (418 amino acids) and size of an epitope (5-6 amino acids), which would give at least 70possibilities in one linear sequence; butof coursethe actual non-denatured native immunogen and antibodyaffinitytendenciesare more complicated, and to be on the safe side I would assume less than this number.

I hope that this will be helpful! Please let me know if you have any other questions, and have a nice day.

Thank you very much for your reply.

Since the kits haven't been tested with non-glycosylated forms of alpha 1 antitrypsin, we don't have any data in this regard and can't say for sure. Since the antibodies used in the kit are polyclonal and raised again the full protein, they will have a range of binding sites along the length of the protein. According to the UniProt entry for this protein, there are 3 known glycosylation sites so there are still many possible antibody binding sites that are common to both glycosylated and non-glycosylated forms. There may be some bias against the non-glycosylated protein, but it's difficult to predict the extent of the bias without more data.

I am sorry that I don't have more information in this regard, but I hope this will be useful. If you have further questions or need anything else, please let me know and I'll be happy to help.

Thank you very much for your call earlier this month and for your patience while I have been working with the lab to get this information for you. I sincerely apologize for the extended delay.

The standard is a native protein isolated from human plasma. It is greater than 95% pure by SDS-PAGE and protein assays. The antibodies were raised against this native, glycosylated protein, so the kit does work with glycosylated samples. The kit is guaranteed to work with cell culture supernatants, urine, milk, and saliva. Both the capture and detection antibodies are polyclonal.

I hope that this information will be useful, and I apologize again for the long dely. Please let me know if you have any further questions or if there is anything else that we can do for you.

Thank you for contacting us.

Unfortunately we have not tested this kit with Plasma samples however keeping in mind the recommended sample type, it is more likely that this kit will be suitable for plasma samples also.

As we have not tested plasma sample so we will not be able to recommend the dilution to use. In similar types of kits we recommend our customer trying several serial dilutions for optimal results and the dilution factor has to be determined by end user.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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