人Alpha 1 Acid Glycoprotein / AGP ELISA试剂盒(AGP) (ab108852)
Key features and details
- Sensitivity: 2 ng/ml
- Range: 3.125 ng/ml - 200 ng/ml
- Sample type: Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Alpha 1 Acid Glycoprotein / AGP ELISA试剂盒(AGP)
参阅全部 Alpha 1 Acid Glycoprotein/AGP 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 6% 批次间 样品 n Mean SD CV% Overall 9.7% -
样品类型
Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
= 2 ng/ml -
范围
3.125 ng/ml - 200 ng/ml -
回收率
= 96 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Alpha 1 Acid Glycoprotein / AGP Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of AGP levels in cell culture supernatant, saliva, milk, and urine.
An Alpha 1 Acid Glycoprotein / AGP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Alpha 1 Acid Glycoprotein / AGP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Alpha 1 Acid Glycoprotein / AGP captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Biotinylated Human Alpha 1 Acid Glycoprotein / AGP Antibody 1 x 60µl 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Alpha 1 Acid Glycoprotein / AGP Microplate (12 x 8 well strips) 1 unit Alpha 1 Acid Glycoprotein / AGP Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
研究领域
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功能
Appears to function in modulating the activity of the immune system during the acute-phase reaction. -
组织特异性
Expressed by the liver and secreted in plasma. -
序列相似性
Belongs to the calycin superfamily. Lipocalin family. -
细胞定位
Secreted. - Information by UniProt
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别名
- A1AG1_HUMAN
- AGP
- AGP 1
see all -
数据库链接
- Entrez Gene: 5004 Human
- Omim: 138600 Human
- SwissProt: P02763 Human
- Unigene: 522356 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108852于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical standard curve
Representative Standard Curve using ab108852.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab108852 被引用在 4 文献中.
- Qaisar R et al. Circulating Biomarkers of Handgrip Strength and Lung Function in Chronic Obstructive Pulmonary Disease. Int J Chron Obstruct Pulmon Dis 15:311-321 (2020). PubMed: 32103930
- Mateos J et al. Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection. Sci Rep 10:3844 (2020). PubMed: 32123229
- Zhan Z et al. Urine a-fetoprotein and orosomucoid 1 as biomarkers of hepatitis B virus-associated hepatocellular carcinoma. Am J Physiol Gastrointest Liver Physiol 318:G305-G312 (2020). PubMed: 31736338
- Chen WQ et al. Polymorphism of ORM1 is associated with the pharmacokinetics of telmisartan. PLoS One 8:e70341 (2013). PubMed: 23940561