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AB313505

人ALDH4A1/P5CDH ELISA试剂盒

Human ALDH4A1/P5CDH ELISA Kit

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Human ALDH4A1/P5CDH ELISA Kit is a Sandwich ELISA for the measurement of Human ALDH4A1/P5CDH in Human in Cell Culture Media, Biofluids samples.

查看别名

ALDH4, P5CDH, ALDH4A1, P5C dehydrogenase, Aldehyde dehydrogenase family 4 member A1, L-glutamate gamma-semialdehyde dehydrogenase

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Sandwich ELISA - Human ALDH4A1/P5CDH ELISA Kit (AB313505)
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Supplier Data

Sandwich ELISA - Human ALDH4A1/P5CDH ELISA Kit (AB313505)

Example of standard curve using ab313505. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Cell culture supernatant, Plasma, Serum

反应种属

Human

检测类型

Sandwich

灵敏度

= 0.48 ng/mL

范围

0.48 - 120 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human ALDH4A1/P5CDH ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ALDH4A1/P5CDH in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ALDH4A1/P5CDH coated on a 96-well plate.
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回收率

[ { "sample": "Cell culture media", "range": "123 - 136 %", "average": "= 131" }, { "sample": "Plasma", "range": "84 - 141 %", "average": "= 100.7" }, { "sample": "Serum", "range": "100 - 104 %", "average": "= 101.1" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313505-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human ALDH4A1/P5CDH Detection Antibody", "number":"AB313505-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313505-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313505-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313505-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ALDH4A1/P5CDH Antibody-coated ELISA Plate", "number":"AB313505-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human ALDH4A1/P5CDH Protein Standard", "number":"AB313505-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313505-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin", "number":"AB313505-CMP03", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ALDH4A1 also known as P5CDH is an enzyme responsible for catalyzing the conversion of pyrroline-5-carboxylate to glutamate in the mitochondria. It has a molecular mass of approximately 65 kDa and plays an essential role in the proline degradation pathway. The enzyme is primarily expressed in the liver kidney and muscle tissues where it supports the metabolism of amino acids and energy production.
Biological function summary

ALDH4A1 is involved in the L-proline degradation pathway converting substrates to downstream metabolic intermediates. While it operates mainly as an individual enzyme it facilitates the integration of amino acid metabolism with cellular energy cycles. A deficiency in ALDH4A1 activity disrupts normal nitrogen balance and mitochondrial function illustrating its relevance to cellular homeostasis.

Pathways

ALDH4A1 plays a significant role in the urea cycle and the proline degradation pathway. It interacts with enzymes like delta-1-pyrroline-5-carboxylate synthetase which provides substrates for its enzymatic activity. These interactions are important for maintaining nitrogen homeostasis and chemical energy balance within cells highlighting its contributions to metabolic circuits.

Mutations and deficiencies in ALDH4A1 lead to hyperprolinemia type II and can contribute to neurological issues. The protein interacts with other cellular components such as ALDH3A2 which also participates in detoxifying reactive aldehydes. Abnormal ALDH4A1 function can exacerbate proline accumulation and neurotoxic effects in affected individuals making it a significant focus for understanding metabolic disorders.
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产品实验方案

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靶点信息

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.
See full target information ALDH4A1
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websiteProtocolBooklet
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搭配使用产品

Primary Antibodies

AB185208

Anti-ALDH4A1/P5CDH antibody [EPR14287]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (AB185208)

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Proteins & Peptides

AB160138

Recombinant Human ALDH4A1/P5CDH protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ALDH4A1/P5CDH protein (GST tag N-Terminus) (AB160138)

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