人Albumin ELISA试剂盒(ab108788)
Key features and details
- Sensitivity: 0.79 ng/ml
- Range: 6.25 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cerebral Spinal Fluid, Milk, Saliva, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Albumin ELISA试剂盒
参阅全部 Albumin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.9% 批次间 样品 n Mean SD CV% Overall 10.4% -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Tissue, Cell Lysate, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.79 ng/ml -
范围
6.25 ng/ml - 100 ng/ml -
回收率
99 %
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检测时间
3h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human Albumin ELISA kit (ab108788) is a sandwich ELISA kit designed for the quantitative measurement of albumin levels in urine, saliva, milk, CSF, cell culture supernatants, cell lysate and tissue samples.
An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.
Get results in 90 minutes with Human Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.
Please note: For both the 10X Diluent N Concentrate and 20X Wash Buffer Concentrate, please ensure to mix well by inversion before use to avoid precipitation.
The concentration of the biotinylated antibody varies by lot: 30X, 40X, 50X, etc. The stock Biotinylated Albumin Antibody must be diluted with 1X Diluent N to prepare 1X Biotinylated Albumin Antibody for use in the assay procedure. Observe the label for the “X” concentration on the vial of Biotinylated Albumin Antibody.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 25X Biotinylated Human Albumin Antibody 1 x 200µl Albumin Microplate (12 x 8 well strips) 1 unit Albumin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
组织特异性
Plasma. -
疾病相关
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻译后修饰
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
细胞定位
Secreted. - Information by UniProt
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别名
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
数据库链接
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
- Unigene: 592379 Human
相关产品
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SimpleStep ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108788于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve for ab108788 Human Albumin ELISA kit
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ab108788 Albumin Human ELISA kitStandard curve with background signal subtracted (duplicates; +/- SD).
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ab108788 Albumin Human ELISA kitConcentration of albumin from differently diluted (in parenthesis) samples, demonstrating a linearity of 84-90% in CSF and 88-111% in milk (duplicates; +/- SD).
数据表及文件
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SDS download
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Datasheet download
文献 (55)
ab108788 被引用在 55 文献中.
- Carbonaro M et al. IL-6-GP130 signaling protects human hepatocytes against lipid droplet accumulation in humanized liver models. Sci Adv 9:eadf4490 (2023). PubMed: 37058568
- Jin Y et al. Adipose-derived stem cells show hepatic differentiation potential and therapeutic effect in rats with acute liver failure. Acta Biochim Biophys Sin (Shanghai) 55:601-612 (2023). PubMed: 37078751
- Wilton DK et al. Microglia and complement mediate early corticostriatal synapse loss and cognitive dysfunction in Huntington's disease. Nat Med 29:2866-2884 (2023). PubMed: 37814059
- Cayley AN et al. Development of a network of carcinogenicity adverse outcome pathways and its employment as an evidence framework for safety assessment. ALTEX N/A:N/A (2022). PubMed: 35575642
- Daley MC et al. Beyond pharmaceuticals: Fit-for-purpose new approach methodologies for environmental cardiotoxicity testing. ALTEX N/A:N/A (2022). PubMed: 35648122