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AB325365

Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent

Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent

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Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human Alanine aminotransferase (ALT) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :29 - 63000 pg/mL with a sensitivity of 14 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent Pending

查看别名

AAT1, GPT1, GPT, Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1

5 Images
Sandwich ELISA - Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (AB325365)
  • sELISA

Lab

Sandwich ELISA - Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (AB325365)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (AB325365)
  • sELISA

Lab

Sandwich ELISA - Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (AB325365)

Interpolated concentration of native ALT was measured in duplicate at different sample concentrations. Undiluted samples are 25% serum, plasma (citrate), and plasma (EDTA). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (AB325365)
  • sELISA

Lab

Sandwich ELISA - Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (AB325365)

Dynamic range comparison between the Chemiluminescent Human ALT SimpleStep ELISA and colorimetric Human ALT SimpleStep ELISA (ab234578). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (AB325365)
  • sELISA

Lab

Sandwich ELISA - Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (AB325365)

Example of Human ALT standard curve ran in chemiluminescent vs. colorimetric assay format in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (AB325365)
  • sELISA

Lab

Sandwich ELISA - Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (AB325365)

Serum of eight individual healthy Human male donors were measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean ALT concentration was determined to be 1,415 pg/mL with a range of 411 – 3,660 pg/mL.

关键信息

检测方法

Luminescent

样品类型

Serum, Citrate plasma, EDTA Plasma, Cell culture media

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 14 pg/mL

范围

29 - 63000 pg/mL

检测时间

55m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (ab325365) is a rapid single-wash sandwich 55-min ELISA to measure Human Alanine aminotransferase (ALT) in Serum, Citrate Plasma, EDTA Plasma, Cell Culture Media with a sensitivity of 14 pg/ml.

How the assay works

Human Alanine aminotransferase (ALT) ELISA Kit, Chemiluminescent (ab325365) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human Alanine aminotransferase (ALT) (ab325365) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Rabbit monoclonal [EPR21068-102] to Alanine aminotransferase 1  - BSA and Azide free (Capture) ab244765

- Rabbit monoclonal [EPR21068-86] to Alanine aminotransferase 1  - BSA and Azide free (Detector) ab245036

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325365-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325365-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325365-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325365-CMP11", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325365-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB325365-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Alanine aminotransferase (ALT) Capture Antibody", "number":"AB325365-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325365-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325365-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Alanine aminotransferase (ALT) Lyophilized Recombinant Protein", "number":"AB325365-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Alanine aminotransferase (ALT) Detector Antibody", "number":"AB325365-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB325365-CMP05", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
See full target information GPT
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websiteProtocolBooklet
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