人AIF Profiling ELISA试剂盒(ab126583)
Key features and details
- Sensitivity: 6 µg/ml
- Range: 6 µg/ml - 250 µg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
概述
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产品名称
人AIF Profiling ELISA试剂盒
参阅全部 AIF 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Cell lysates 4 3.6% 批次间 样品 n Mean SD CV% Cell lysates 3 11.4% -
样品类型
Tissue Extracts, Cell Lysate -
检测类型
Sandwich (qualitative) -
灵敏度
> 6 µg/ml -
范围
6 µg/ml - 250 µg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
产品概述
Apoptosis-inducing factor is a mitochondrial enzyme with probable oxidoreductase activity which functions to regulate Complex I activity in the mitochondrial membrane. When mitochondria are damaged, as in apoptosis, AIF can migrate to the cytosol and nucleus. AIF in the nucleus signals chromatin condensation and DNA fragmentation. This method of programmed cell death (apoptosis) does not require caspase activation.
In addition to its role in apoptosis defects in AIF1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6). COXPD6 is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
Get results in 90 minutes with Human AIF ELISA Kit (Apoptosis-Inducing Factor 1) (ab184858) from our SimpleStep ELISA® range.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X AIF Detector Antibody 1 x 1ml 10X Blocking Buffer 1 x 6ml 10X HRP Label 1 x 1ml 20X Buffer 1 x 20ml AIF Microplate (12 x 8 antibody coated well strips) 1 unit Extraction Buffer (ab260490) 1 x 15ml HRP Development Solution 1 x 12ml -
研究领域
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功能
Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. -
疾病相关
Defects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting. -
序列相似性
Belongs to the FAD-dependent oxidoreductase family. -
翻译后修饰
Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner. -
细胞定位
Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region. - Information by UniProt
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别名
- AIFM1
- AIFM1_HUMAN
- Apoptosis inducing factor
see all -
数据库链接
- Entrez Gene: 51060 Human
- Entrez Gene: 9131 Human
- Omim: 300169 Human
- SwissProt: O95831 Human
- Unigene: 424932 Human
- Unigene: 476033 Human
相关产品
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Related Products
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SimpleStep ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126583于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Immunocytochemistry/ Immunofluorescence - AIF Human Profiling ELISA Kit (ab126583)Immunocytochemistry analysis of normal untreated HeLa cells with the capture antibody in this kit, ab110327, at 5µg/ml shows staining of AIF is located exclusively to mitochondria. Nuclei were stained with DAPI.
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Western blot - AIF Human Profiling ELISA Kit (ab126583) -
ELISA - AIF Human Profiling ELISA Kit (ab126583)Example serially titrated control sample of HeLa cell extract in the working range of the assay.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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