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AB246536

人4-1BBL ELISA试剂盒

Human 4-1BBL ELISA Kit

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Human 4-1BBL ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Human 4-1BBL with a sensitivity of 2.2 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab327891

查看别名

Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, TNFSF9

4 Images
ELISA - Human 4-1BBL ELISA Kit (AB246536)
  • ELISA

Supplier Data

ELISA - Human 4-1BBL ELISA Kit (AB246536)
Sandwich ELISA - Human 4-1BBL ELISA Kit (AB246536)
  • sELISA

Supplier Data

Sandwich ELISA - Human 4-1BBL ELISA Kit (AB246536)

Interpolated concentrations of native 4-1BBL in human cell culture supernatant samples.

The concentrations of 4-1BBL were measured in duplicates, interpolated from the 4-1BBL standard curves and corrected for sample dilution. Undiluted samples are as follows : Raji (PMA treated) cell culture supernatant 50% and Raji (untreated) cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean 4-1BBL concentration was determined to be 143.7 pg/mL in Raji (PMA treated) cell culture supernatant, and 59.2 pg/mL in Raji (untreated) cell culture supernatant.

Sandwich ELISA - Human 4-1BBL ELISA Kit (AB246536)
  • sELISA

Lab

Sandwich ELISA - Human 4-1BBL ELISA Kit (AB246536)

Example of Human 4-1BBL standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human 4-1BBL ELISA Kit (AB246536)
  • sELISA

Supplier Data

Sandwich ELISA - Human 4-1BBL ELISA Kit (AB246536)

Example of human 4-1BBL standard curve in Sample Diluent NS.

The 4-1BBL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Citrate plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 2.2 pg/mL

范围

6.25 - 400 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

Assay Kits

AB327891

Human 4-1BBL ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA|Recombinant

Sandwich ELISA - Human 4-1BBL ELISA Kit, Chemiluminescent (AB327891)

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AB264618

Human IL-15RA ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

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Assay Kits

AB213842

Human TNFSF4 ELISA Kit (OX40L)

Sandwich ELISA - Human TNFSF4 ELISA Kit (OX40L) (AB213842)

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Assay Kits

AB196268

Human CD40L ELISA Kit (TNFSF5)

Recombinant|SimpleStep

ELISA - Human CD40L ELISA Kit (TNFSF5) (AB196268)

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Assay Kits

AB264621

Human CD70 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD70 ELISA Kit (AB264621)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human 4-1BBL ELISA Kit ab246536 is a rapid single-wash 90-min ELISA kit to measure Human 4-1 BBL in Citrate plasma, Cell culture supernatant, Serum. This SimpleStep ELISA® sensitivity is 5.64 pg/mL.

How the assay works

Human 4-1 BBL SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit is designed for the quantification of Human 4-1BBL. The standard protein in this kit is the extracellular domain of Human 4-1BBL. Native signal was detected in cell culture supernatant. Spiked protein experiments were used to validate serum and plasma (citrate) sample types. Neat pooled serum and plasma (citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay. Saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit. This kit is incompatible with plasma (EDTA) and plasma (heparin) samples.

Human 4-1BBL ELISA Kit ab246536 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

Save your precious samples

Our 384-well Human 4-1BBL ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human 4-1BBL Antibody Pair - BSA and Azide free ab256681
- Anti-4-1BBL antibody [EPR21995-69] - BSA and Azide free (Capture) ab259609
- Anti-4-1BBL antibody [EPR21995-47] - BSA and Azide free (Detector) ab259610

Related and recommended products

Human 4-1BBL ELISA Kit ab246536 is commonly used to study immune cell activation and regulation:

Other kits for measuring immune response include:

- Human CD40L ELISA kit ab99991
- Human TNFSF4 ELISA kit (OX40L) ab213842
- Human TNF alpha ELISA Kit ab181421

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精确度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "99 - 104 %", "average": "= 101" }, { "sample": "Serum", "range": "86 - 89 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "90 - 94 %", "average": "= 92" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246536-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246536-CMP03" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246536-CMP10" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246536-CMP04" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246536-CMP07" }, { "size":"1 x 600 µL", "name":"10X Human 4-1BBL Detector Antibody", "number":"AB246536-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB246536-CMP01" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246536-CMP02" }, { "size":"2 x 1 Vial", "name":"Human 4-1BBL Lyophilized Recombinant Protein", "number":"AB246536-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human 4-1BBL Capture Antibody", "number":"AB246536-CMP05" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB246536-CMP09" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab246536-10x96Tests", "assetComponentDetails": [ { "size":"1 x 6000 µL", "name":"10X Human 4-1BBL Detector Antibody", "number":"AB246536-CMP06" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB246536-CMP04" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB246536-CMP07" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB246536-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246536-CMP03" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246536-CMP10" }, { "size":"1 x 6000 µL", "name":"10X Human 4-1BBL Capture Antibody", "number":"AB246536-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB246536-CMP09" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246536-CMP02" }, { "size":"2 x 10 Vial", "name":"Human 4-1BBL Lyophilized Recombinant Protein", "number":"AB246536-CMP08" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab246536-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB246536-CMP07" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246536-CMP10" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB246536-CMP11" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246536-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human 4-1BBL Capture Antibody", "number":"AB246536-CMP05" }, { "size":"1 x 600 µL", "name":"10X Human 4-1BBL Detector Antibody", "number":"AB246536-CMP06" }, { "size":"2 x 1 Vial", "name":"Human 4-1BBL Lyophilized Recombinant Protein", "number":"AB246536-CMP08" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB246536-CMP04" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB246536-CMP01" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB246536-CMP09" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab246536-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB246536-CMP09" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB246536-CMP01" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB246536-CMP07" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246536-CMP02" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246536-CMP10" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB246536-CMP04" }, { "size":"1 x 600 µL", "name":"10X Human 4-1BBL Capture Antibody", "number":"AB246536-CMP05" }, { "size":"2 x 1 Vial", "name":"Human 4-1BBL Lyophilized Recombinant Protein", "number":"AB246536-CMP08" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB246536-CMP11" }, { "size":"1 x 600 µL", "name":"10X Human 4-1BBL Detector Antibody", "number":"AB246536-CMP06" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab246536-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB246536-CMP09" }, { "size":"1 x 6000 µL", "name":"10X Human 4-1BBL Capture Antibody", "number":"AB246536-CMP05" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB246536-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246536-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB246536-CMP07" }, { "size":"2 x 10 Vial", "name":"Human 4-1BBL Lyophilized Recombinant Protein", "number":"AB246536-CMP08" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB246536-CMP01" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246536-CMP02" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246536-CMP03" }, { "size":"1 x 6000 µL", "name":"10X Human 4-1BBL Detector Antibody", "number":"AB246536-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246536-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB246536-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246536-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246536-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246536-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246536-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246536-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB246536-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human 4-1BBL Capture Antibody", "number":"AB246536-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human 4-1BBL Lyophilized Recombinant Protein", "number":"AB246536-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human 4-1BBL Detector Antibody", "number":"AB246536-CMP06", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
See full target information TNFSF9
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websiteProtocolBooklet
en
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