HSP70 High Sensitivity ELISA试剂盒(ab133061)
Key features and details
- Sensitivity: 90 pg/ml
- Range: 0.2 ng/ml - 12.5 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat, Human
概述
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产品名称
HSP70 High Sensitivity ELISA试剂盒
参阅全部 HSP70 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Buffer 20 5.11ng/ml 3.9% Buffer 20 2.19ng/ml 11.4% Buffer 20 0.99ng/ml 5.9% 批次间 样品 n Mean SD CV% Buffer 4.98ng/ml 12.8% Buffer 2.63ng/ml 13.7% Buffer 1.08ng/ml 19.1% -
样品类型
Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
90 pg/ml -
范围
0.2 ng/ml - 12.5 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 94.2 % - % Plasma 79.3 % - % -
检测时间
4h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse, Rat, Human -
产品概述
Abcam’s HSP70 High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of HSP70 in serum and plasma samples from human, mouse and rat origin. It does not detect other Hsp70 family members such as Hsc70 (Hsp73), Grp78, DnaK (E. coli), or Hsp71 (M. tuberculosis).
A HSP70 mouse monoclonal antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A HSP70 polyclonal antibody is then added, incubated and washed. An HRP conjugated anti-IgG antibody is then added, incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.
Get higher sensitivity in only 90 minutes with Human HSP70 ELISA Kit (ab187399) from our SimpleStep ELISA® range.
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说明
HSP70 is a molecular chaperone whose expression is induced upon exposure of the cell or organism to conditions of stress. It prevents protein aggregation and promotes the refolding of proteins that become damaged in response to environmental insults, pathogens, and disease. Its activity is essential for cellular survival and recovery under stress conditions, as well as for the maintenance of normal cellular function under non-stress conditions. HSP70 has been implicated to play a role in a variety of disease and physiological processes such as hyperthermia, hypertension, toxic exposure to chemical agents, hypoxia, ischemia, inflammation, autoimmunity, apoptosis, cancer, organ transplantation, and bacterial and viral infections. HSP70 is also a key regulator of many normal physiological processes including aging, spermatogenesis, menstruation, and physical activity such as exercise. The HSP70 high sensitivity ELISA kit is designed to evaluate and monitor HSP70 in these processes, providing a key research tool to understand the role of HSP70 in physiology and disease.
Cross reactivities:
Compound Cross reactivity HSP70 (Human) 100% HSP70 (rat) 117.6% HSP70B’ (Human) 5.4% HSP70 (salmon) 0.8% DnaK (E.coli) 0.5% Hsc70 (bovine) <0.016% Grp78 (hamster) <0.016% Hsp71 (M. tuberculosis) <0.016% Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 100ml Anti-HSP70 Microplate (12 x 8 wells) 1 unit Assay Buffer 28 1 x 50ml Hsp70 Antibody 1 x 10ml HSP70 Horseradish Peroxidase Conjugate 1 x 10ml Plate Sealer 3 units Recombinant HSP70 Standard 1 x 25µl Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
研究领域
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细胞定位
Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs -
别名
- DnaK type molecular chaperone HSP70 1
- Heat shock 70 kDa protein 1/2
- Heat shock 70 kDa protein 1A/1B
see all -
数据库链接
- Entrez Gene: 3303 Human
- Entrez Gene: 193740 Mouse
- Entrez Gene: 24472 Rat
- Omim: 140550 Human
- SwissProt: P08107 Human
- SwissProt: P0DMV8 Human
- SwissProt: P0DMV9 Human
- SwissProt: Q61696 Mouse
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133061于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab133061 被引用在 6 文献中.
- Fu W et al. Nav1.7 as a chondrocyte regulator and therapeutic target for osteoarthritis. Nature 625:557-565 (2024). PubMed: 38172636
- Xie Y et al. Exogenous recombinant Hsp70 attenuates sevoflurane anesthesia-induced cognitive dysfunction in aged mice. Brain Behav 13:e2861 (2023). PubMed: 36573756
- Tessier SN et al. Partial freezing of rat livers extends preservation time by 5-fold. Nat Commun 13:4008 (2022). PubMed: 35840553
- Shaw J et al. Effectiveness of short-term isothermic-heat acclimation (4 days) on physical performance in moderately trained males. PLoS One 17:e0270093 (2022). PubMed: 36395344
- Mouratidis PXE & Ter Haar G HSP90 inhibition acts synergistically with heat to induce a pro-immunogenic form of cell death in colon cancer cells. Int J Hyperthermia 38:1443-1456 (2021). PubMed: 34612127
- Gholamnezhadjafari R et al. Innate inflammatory gene expression profiling in potential brain-dead donors: detailed investigation of the effect of common corticosteroid therapy. Innate Immun 23:440-448 (2017). PubMed: 28504607