马TIMP2 ELISA试剂盒(ab272033)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 16.38 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse, Human
概述
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产品名称
马TIMP2 ELISA试剂盒
参阅全部 TIMP2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
4 pg/ml -
范围
16.38 pg/ml - 4000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 141.3 131% - 149% Serum 141.2 135% - 148% Plasma 143.3 137% - 149% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Horse, Human -
产品概述
Horse TIMP-2 ELISA Kit is designed for the quantitative determination of Horse TIMP-2 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for TIMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Detects horse and human TIMP2.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Horse TIMP2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Horse TIMP2 Detection Antibody 2 vials Horse TIMP2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. -
序列相似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻译后修饰
The activity of TIMP2 is dependent on the presence of disulfide bonds. -
细胞定位
Secreted. - Information by UniProt
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别名
- CSC 21K
- CSC-21K
- CSC21K
see all -
数据库链接
- Entrez Gene: 100034134 Horse
- Entrez Gene: 7077 Human
- Omim: 188825 Human
- SwissProt: O77717 Horse
- SwissProt: P16035 Human
- Unigene: 633514 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272033于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Horse TIMP-2 Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
Horse TIMP-2 ELISA kit (ab272033) Standard curve.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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