马IL-1 alpha ELISA试剂盒(ab272030)
Key features and details
- Sensitivity: 0.82 pg/ml
- Range: 0.82 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse
概述
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产品名称
马IL-1 alpha ELISA试剂盒
参阅全部 IL-1 alpha 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.82 pg/ml -
范围
0.82 pg/ml - 200 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 141.2 136% - 144% Serum 143.5 141% - 146% Plasma 145.2 141% - 148% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Horse -
产品概述
Horse IL-1alpha ELISA Kit is designed for the quantitative determination of Horse IL-1alpha in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for IL-1alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse IL-1alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Horse IL-1 alpha coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Horse IL-1 alpha Detection Antibody 2 vials Horse IL-1 alpha Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
序列相似性
Belongs to the IL-1 family. -
结构域
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
细胞定位
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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别名
- BAF
- FAF
- Hematopoietin 1
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272030于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Horse IL-1alpha Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
Horse IL-1alpha ELISA kit (ab272030) Standard curve.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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