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AB316915

GFP ELISA试剂盒- Extracellular

GFP ELISA Kit- Extracellular

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GFP ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify GFP with a sensitivity of 1.813 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

See more GFP assay kits and antibodies

查看别名

Green fluorescent protein, GFP

3 Images
Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)

Interpolated concentrations of spiked untreated eGFP in HeLa supernatant and spiked denatured eGFP in HeLa supernatant

Recombinant untreated and 2% SDS-denatured eGFP were diluted 1 : 20 in 50% Hela cell culture supernatant (prepared in Sample Diluent NS) to prepare the undiluted sample, and then diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)

Example of GFP standard curve in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)

Standard curve comparison between the original GFP SimpleStep ELISA (ab171581) and current GFP SimpleStep ELISA (ab316915).

关键信息

检测方法

Colorimetric

样品类型

Cell culture supernatant

反应种属

Aequorea victoria

检测类型

Sandwich

Quantitative
灵敏度

= 1.813 pg/mL

范围

9.375 - 600 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Assay Kits

AB221829

mCherry ELISA Kit

Recombinant|SimpleStep

ELISA - mCherry ELISA Kit (AB221829)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

GFP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GFP protein in Cell culture supernatant. Quantitate  GFP with 1.813 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "94 - 104 %", "average": "= 98" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316915-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316915-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB316915-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316915-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316915-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316915-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X GFP Capture Antibody", "number":"AB316915-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316915-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB316915-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"GFP Lyophilized Recombinant Protein", "number":"AB316915-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X GFP Detector Antibody", "number":"AB316915-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316915-CMP10", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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websiteProtocolBooklet
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Abcam Product Promise

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详情请参阅我们的条款与条件。

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