Feline IL-2 ELISA试剂盒(ab273227)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 24.58 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cat
概述
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产品名称
Feline IL-2 ELISA试剂盒
参阅全部 IL-2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
25 pg/ml -
范围
24.58 pg/ml - 6000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 112.5 84% - 149% Plasma 110.4 92% - 148% Cell culture media 118.9 92% - 147% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Cat -
产品概述
Feline IL-2 ELISA Kit ELISA Kit (ab273227) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline IL-2 ELISA Kit in serum (Feline IL-2 concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Feline IL-2 ELISA Kit coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Feline IL-2 ELISA Kit antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Feline IL-2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Feline IL-2 Antibody 2 vials Feline IL-2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
疾病相关
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
序列相似性
Belongs to the IL-2 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Aldesleukin
- IL 2
- IL-2
see all -
数据库链接
- Entrez Gene: 751114 Cat
- SwissProt: Q07885 Cat
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273227于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Feline IL-2 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab273227 尚未被引用在任何文献中。