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AB273228

Feline IL-10 ELISA试剂盒

Feline IL-10 ELISA Kit

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Feline IL-10 ELISA Kit is a Sandwich ELISA for the measurement of Feline IL-10 in Cat in Biofluids, Cell Culture Media samples.

查看别名

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

1 Images
Sandwich ELISA - Feline IL-10 ELISA Kit (AB273228)
  • sELISA

Supplier Data

Sandwich ELISA - Feline IL-10 ELISA Kit (AB273228)

This standard curve is for demonstration only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Cat

检测类型

Sandwich

Quantitative
灵敏度

= 0.2 ng/mL

范围

0.205 - 50 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Feline IL-10 ELISA Kit (ab273228) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of feline IL-10 in serum (feline IL-10 concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for feline IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- feline IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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回收率

[ { "sample": "Serum", "range": "71 - 100 %", "average": "= 82.07" }, { "sample": "Plasma", "range": "73 - 104 %", "average": "= 86.6" }, { "sample": "Cell culture media", "range": "87 - 111 %", "average": "= 99.06" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273228-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273228-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273228-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273228-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273228-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Feline IL-10 coated Microplate (12 x 8 wells)", "number":"AB273228-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB273228-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Feline IL-10 Antibody", "number":"AB273228-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Feline IL-10 Standard (Lyophilized)", "number":"AB273228-CMP01", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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产品实验方案

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靶点信息

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information IL10
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websiteProtocolBooklet
en
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