ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) In-Cell ELISA试剂盒(ab126423)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Colorimetric
- Sample type: Adherent cells
- Reacts with: Mouse, Rat, Human
概述
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产品名称
ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) In-Cell ELISA试剂盒 -
检测方法
Colorimetric -
样品类型
Adherent cells -
检测类型
Cell-based (qualitative) -
检测时间
5h 10m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse, Rat, Human -
产品概述
ab126423 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (Thr202/Tyr204) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured Human, Mouse and Rat cell lines. By determining ERK1/2 protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.
In the ERK1/2 (Thr202/Tyr204) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, anti-Phospho-ERK1/2 (Thr202/Tyr204) or anti-ERK1/2 is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-mouse IgG is added to the wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests HRP-conjugated Anti-Mouse IgG Concentrate 1 x 10µl Blocking Buffer Concentrate (5X) 1 x 20ml Fixing Solution 1 x 30ml Uncoated 96-well Microplate 1 unit Mouse anti-ERK1/2 Concentrate (1,000X) 1 x 7µl Mouse anti-Phospho-ERK1/2 (Thr202/Tyr204) Concentrate (1,000X) 1 x 7µl Quenching Buffer Concentrate (30x) 1 x 2ml Stop Solution 1 x 14ml TMB One-Step Substrate Reagent 1 x 12ml Wash Buffer A Concentrate (20X) 1 x 30ml Wash Buffer B Concentrate (20X) 1 x 30ml -
研究领域
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细胞定位
ERK2: Nucleus. -
数据库链接
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
- Omim: 601795 Human
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126423于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
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ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) In-Cell ELISA Kit (ab126423)
A431 cells were stimulated by different concentrations of EGF for 10 min at 37°C.
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Western blot - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) In-Cell ELISA Kit (ab126423)Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells. Phospho-ERK1/2 (Thr202/Tyr204) and ERK1/2 antibodies were used in both detection assays.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab126423 被引用在 2 文献中.
- Huang Y et al. miR-101 regulates the cell proliferation and apoptosis in diffuse large B-cell lymphoma by targeting MEK1 via regulation of the ERK/MAPK signaling pathway. Oncol Rep 41:377-386 (2019). PubMed: 30365139
- Tang F et al. CT45A1 siRNA silencing suppresses the proliferation, metastasis and invasion of lung cancer cells by downregulating the ERK/CREB signaling pathway. Mol Med Rep 16:6708-6714 (2017). PubMed: 28901509