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AB176640

ERK1/2 (pT202/Y204) ELISA试剂盒

ERK1/2 (pT202/Y204) ELISA Kit

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(1 Review)

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(16 Publications)

ERK1/2 (pT202/Y204) ELISA Kit is a single-wash 90-min Simplestep used to quantify ERK1/2 (pT202/Y204) with a sensitivity of 0.1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 10 citations

查看别名

ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK

6 Images
ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)
  • ELISA

Supplier Data

ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)
Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)
  • sELISA

Supplier Data

Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)

Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of ERK1/2 (pT202/Y204) are normalized and plotted.

Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)
  • sELISA

Supplier Data

Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)

Typical recombinant protein standard curve.

Example of a typical ERK1/2 (pT202/Y204) recombinant protein standard curve. The proportion of total protein that is phosphorylated is unknown - data is indicative only. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)
  • sELISA

Supplier Data

Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)

Typical cell lysate dilution series.

Example of a typical ERK1/2 (pT202/Y204) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)
  • sELISA

Supplier Data

Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)

Comparison of total ERK1/2 expression in different cell lines.

Cell line analysis for Total ERK1/2 from 100 μg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).

Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)
  • sELISA

Supplier Data

Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)

ERK1/2 (pT202/Y204) phosphorylation in response to EGF treatment.

Induction of ERK1/2 (pT202/Y204) phosphorylation in MCF-7 cells in response to EGF treatment. MCF-7 cells were cultured in 96-well tissue culture plates, serum starved and treated (10 min) with a dose-range of EGF before cell lysis. Data from quadruplicate measurements of ERK1/2 (pT202/Y204) are plotted and compared against Total ERK1/2 protein levels. Comparative ERK1/2 (pT202/Y204) and ERK1/2 (Total) data also shown by Western Blot.

关键信息

检测方法

Colorimetric

样品类型

Tissue Homogenate, Cell Lysate

反应种属

Mouse, Human

检测类型

Semi-quantitative

灵敏度

= 0.1 ng/mL

范围

0.2 - 20 ng/mL

检测时间

1h 30m

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

ERK1/2 (pT202/Y204) ELISA Kit ab176640 is a rapid single-wash 90-min Other Immunoassay to measure ERK1/2 (pT202/Y204) in cell lysate, tissue homogenate. This SimpleStep sensitivity is 0.1 ng/mL.

How the assay works

ERK1/2 (pT202/Y204) SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

ERK1/2 (pT202/Y204) ELISA Kit ab176640 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "A431 Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.3" }, { "reproducibilityType": "Intra", "sample": "A431 Extract", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ERK1 and ERK2 also known as MAPK3 and MAPK1 respectively are protein kinases involved in the mitogen-activated protein kinase (MAPK) signaling pathway. They share high sequence identity and exhibit similar functions. ERK1 has a molecular weight of approximately 44 kDa while ERK2 weighs around 42 kDa. Both are expressed ubiquitously in various tissues playing roles in diverse cellular processes. These proteins often detected through ERK1/2 western blot analyses present similar ERK protein size and ERK molecular weight characteristics relevant during protein expression studies.
Biological function summary

ERK1 and ERK2 play key roles in cell proliferation differentiation and survival. They form part of a cascade that includes upstream activators such as MEK1/2 and downstream targets including transcription factors. As components of the MAPK signaling complex ERK1/2 regulate gene expression through phosphorylation events impacting cellular responses to various stimuli. Their activation often hinges on growth factors cytokines and stress signals facilitating cellular adaptation to environmental changes.

Pathways

Regarding pathways ERK1/2 sit within the MAPK/ERK pathway and are significant in the Ras/Raf/MEK/ERK cascade one of the foremost signaling mechanisms in cells. They interact with several proteins including Ras and Raf which modulate their activation. This pathway is important for transmitting signals from the cell surface to the DNA in the cell nucleus impacting gene regulation and cell fate decisions. ERK1/2 proteins therefore serve as critical nodes linking extracellular signals to cellular responses ensuring balanced cell function.

ERK1/2 play significant roles in cancer and neurodegenerative diseases. Their overactivation is often linked to oncogenic processes contributing to cell proliferation in cancers. Mutations or dysregulation within the MAPK/ERK pathway including ERK1/2 frequently associate with tumorigenesis. Additionally in neurodegenerative disorders like Alzheimer's disease altered ERK1/2 activity may impact neuronal survival and function often through interaction with amyloid-beta and tau proteins further illustrating their importance in disease pathophysiology.
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产品实验方案

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靶点信息

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed : 34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10) (PubMed : 35216969). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
See full target information MAPK3 phospho Y204 + T202
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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Disease models & mechanisms 16: PubMed37021539

2023

Vemurafenib improves muscle histopathology in a mouse model of LAMA2-related congenital muscular dystrophy.

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Ariany Oliveira-Santos,Marisela Dagda,Jennifer Wittmann,Robert Smalley,Dean J Burkin

Experimental and therapeutic medicine 25:260 PubMed37153901

2023

ADAM metallopeptidase domain 10 knockdown enables podocytes to resist high glucose stimulation by inhibiting pyroptosis via MAPK pathway.

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Species

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Chunjie Sui,Dan Zhou

Frontiers in immunology 13:1078678 PubMed36505403

2022

Pro-resolving and anti-arthritic properties of the MC selective agonist PL8177.

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Species

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Jose Garrido-Mesa,Bethan Lynne Thomas,John Dodd,Carl Spana,Mauro Perretti,Trinidad Montero-Melendez

Frontiers in pharmacology 12:720173 PubMed35095479

2022

AKT-AMPKα-mTOR-dependent HIF-1α Activation is a New Therapeutic Target for Cancer Treatment: A Novel Approach to Repositioning the Antidiabetic Drug Sitagliptin for the Management of Hepatocellular Carcinoma.

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Eslam E Abd El-Fattah,Sameh Saber,Mahmoud E Youssef,Hanan Eissa,Eman El-Ahwany,Noha A Amin,Mohammed Alqarni,Gaber El-Saber Batiha,Ahmad J Obaidullah,Mohamed M Y Kaddah,Ahmed Gaafar Ahmed Gaafar,Ahmed A E Mourad,Gomaa Mostafa-Hedeab,Amir Mohamed Abdelhamid

Oral diseases 29:528-541 PubMed34181793

2021

Surfactin induces autophagy, apoptosis, and cell cycle arrest in human oral squamous cell carcinoma.

Applications

Unspecified application

Species

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Thi Thuy Tien Vo,Yinshen Wee,Hsin-Chung Cheng,Ching-Zong Wu,Yuh-Lien Chen,Vo Phuoc Tuan,Ju-Fang Liu,Wei-Ning Lin,I-Ta Lee

Frontiers in psychiatry 12:678090 PubMed34093287

2021

GABA Alpha 2,3 Modulation Improves Select Phenotypes in a Mouse Model of Fragile X Syndrome.

Applications

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Species

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Tori L Schaefer,Amy A Ashworth,Durgesh Tiwari,Madison P Tomasek,Emma V Parkins,Angela R White,Andrew Snider,Matthew H Davenport,Lindsay M Grainger,Robert A Becker,Chandler K Robinson,Rishav Mukherjee,Michael T Williams,Jay R Gibson,Kimberly M Huber,Christina Gross,Craig A Erickson

Frontiers in cellular neuroscience 14:189 PubMed32774242

2020

Potential Involvement of Adiponectin Signaling in Regulating Physical Exercise-Elicited Hippocampal Neurogenesis and Dendritic Morphology in Stressed Mice.

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Species

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Pingjie Wang,Yiyao Liang,Kai Chen,Suk-Yu Yau,Xin Sun,Kenneth King-Yip Cheng,Aimin Xu,Kwok-Fai So,Ang Li

Molecular medicine reports 22:67-76 PubMed32377748

2020

miR‑133b affects cell proliferation, invasion and chemosensitivity in renal cell carcinoma by inhibiting the ERK signaling pathway.

Applications

Unspecified application

Species

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Yuan Xu,Yuan Ma,Xiao-Ling Liu,Sheng-Li Gao

Oncology reports 41:2967-2974 PubMed30864727

2019

Downregulation of estrogen receptor β inhibits lung adenocarcinoma cell growth.

Applications

Unspecified application

Species

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Wenjuan Chen,Bo Xin,Hailin Pang,Le Han,Weiwei Shen,Zheng Zhao,Lian Duan,Peipei Cao,Lili Liu,Helong Zhang

Bratislavske lekarske listy 119:757-761 PubMed30686014

2019

Crocin and voluntary exercise promote heart angiogenesis through Akt and ERK1/2 signalling in type 2 diabetic rats.

Applications

Unspecified application

Species

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H Dariushnejad,M Mohammadi,V Ghorbanzadeh
View all publications
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