Anti-Epstein Barr virus IgA ELISA试剂盒(EBV-VCA) (ab178660)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
概述
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产品名称
Anti-Epstein Barr virus IgA ELISA试剂盒(EBV-VCA) -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Pos. Serum 8 4.8% 批次间 样品 n Mean SD CV% Pos. Serum 3 8.4% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Indirect -
实验步骤
Multiple steps standard assay -
产品概述
Abcam’s anti-Epstein Barr virus (EBV-VCA) IgA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Epstein Barr virus in Human serum and plasma.
A 96-well plate has been precoated with Epstein Barr Virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized with Epstein Barr Virus antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of with Epstein Barr Virus antigens IgA sample captured in plate.
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经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit Epstein Barr Virus (IgA) Coated Microplate (12 x 8 wells) 1 unit Epstein Barr Virus Anti-IgA HRP Conjugate 1 x 20ml Epstein Barr Virus IgA Cut-off Control 1 x 3ml Epstein Barr Virus IgA Negative Control 1 x 2ml Epstein Barr Virus IgA Positive Control 1 x 2ml IgA Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178660于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
图片
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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