Endothelin 1 ELISA试剂盒(ab133030)
Key features and details
- Sensitivity: 0.41 pg/ml
- Range: 0.78 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Pig
概述
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产品名称
Endothelin 1 ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Buffer 20 35.9pg/ml 6.7% Buffer 20 2.3pg/ml 8.9% Buffer 20 1.1pg/ml 8.8% 批次间 样品 n Mean SD CV% Buffer 20 35.1pg/ml 8.3% Buffer 20 2.5pg/ml 5.9% Buffer 20 1.2pg/ml 15.3% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.41 pg/ml -
范围
0.78 pg/ml - 100 pg/ml -
检测时间
2h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse, Rat, Rabbit, Cow, Dog, Human, Pig -
产品概述
Endothelin 1 ELISA kit (ET-1) is designed for the accurate quantitative measurement of Endothelin 1 in serum, plasma and culture fluids. ET-1 has identical amino acid sequence for Human, mouse, rat, cow, dog, pig, and rabbit.
Endothelin 1 specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated I Endothelin 1 detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the Endothelin 1 amount of sample captured in plate.
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说明
Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.
Cross Reactivity
Compound Cross Reactivity Endothelin 1 (ET-1) 100 ET-2 21 ET-3 3.6 Human Big ET-1 <0.1 Rat Big ET-1 <0.1 Human Big ET-2 <0.1 Human Big ET-3 Amide <0.1 Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 100ml Antibody Diluent 1 x 10ml Assay Buffer 17 1 x 100ml Endothelin 1 Antibody Concentrate 1 x 100µl Endothelin 1 Standard 1 x 0.25ml Mouse monoclonal Endothelin 1 Microplate (12 x 8 wells) 1 unit Plate Sealer 3 units Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
研究领域
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相关性
Endothelins are endothelium-derived vasoconstrictor peptides. -
细胞定位
Secreted -
别名
- Big endothelin 1
- ET1
- HDLCQ7
see all -
数据库链接
- Entrez Gene: 281137 Cow
- Entrez Gene: 403424 Dog
- Entrez Gene: 1906 Human
- Entrez Gene: 13614 Mouse
- Entrez Gene: 396915 Pig
- Entrez Gene: 100009270 Rabbit
- Entrez Gene: 24323 Rat
- Omim: 131240 Human
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133030于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
图片
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab133030 被引用在 31 文献中.
- Duangrat R et al. Dual Blockade of TGF-β Receptor and Endothelin Receptor Synergistically Inhibits Angiotensin II-Induced Myofibroblast Differentiation: Role of AT1R/Gαq-Mediated TGF-β1 and ET-1 Signaling. Int J Mol Sci 24:N/A (2023). PubMed: 37108136
- Rivera A et al. Blockade of the mineralocorticoid receptor improves markers of human endothelial cell dysfunction and hematological indices in a mouse model of sickle cell disease. FASEB J 37:e23092 (2023). PubMed: 37482902
- Al-Kuraishy HM et al. The Prospective Effect of Allopurinol on the Oxidative Stress Index and Endothelial Dysfunction in Covid-19. Inflammation 45:1651-1667 (2022). PubMed: 35199285
- Herrera VLM et al. A targetable 'rogue' neutrophil-subset, [CD11b+DEspR+] immunotype, is associated with severity and mortality in acute respiratory distress syndrome (ARDS) and COVID-19-ARDS. Sci Rep 12:5583 (2022). PubMed: 35379853
- Abu Helal R et al. Aortic Fibrosis in Insulin-Sensitive Mice with Endothelial Cell-Specific Deletion of Ceacam1 Gene. Int J Mol Sci 23:N/A (2022). PubMed: 35457157