AB133030

Endothelin 1 ELISA试剂盒

Name: Endothelin 1 ELISA试剂盒 (ab133030)| Abcam中文官网
Brand: Abcam Limited
SKU: AB133030
Price: 11096 CNY
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Endothelin 1 ELISA Kit

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(31 Publications)

Endothelin 1 ELISA Kit is a sandwich ELISA designed to quantify Endothelin 1 with a sensitivity of 0.41 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.78 - 100 pg/mL
- Cited in over 20 publications

查看别名

Endothelin-1, Preproendothelin-1, PPET1, EDN1

2 Images

Sandwich ELISA - Endothelin 1 ELISA Kit (AB133030)

sELISA

Supplier Data

Sandwich ELISA - Endothelin 1 ELISA Kit (AB133030)

Representative Standard Curve using ab133030.

sELISA

Lab

Sandwich ELISA - Endothelin 1 ELISA Kit (AB133030)

Sample results.

Endothelin 1 measured in biological fluids of healthy species showing quantity (pg) per mL of tested sample. Results obtained from samples that were not extracted. Samples diluted 1-9 fold.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Mouse, Rat, Rabbit, Cow, Dog, Human, Pig

检测类型

Sandwich (quantitative)

灵敏度

= 0.41 pg/mL

范围

0.78 - 100 pg/mL

检测时间

检测平台

Microplate

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反应性数据

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产品详情

Endothelin 1 ELISA Kit ab133030 is a sandwich ELISA to measure Endothelin 1 in serum, plasma, cell culture supernatant with a sensitivity of 0.41 pg/ml.

How the assay works

Endothelin 1 specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated I Endothelin 1 detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the Endothelin 1 amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Endothelin 1 ELISA Kit ab133030 protocol summary

1. Add standard or sample to each well used. Incubate wells.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate wells.
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well. Immediately begin recording the color development.

Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.

Cross Reactivity

Compound	Cross Reactivity
Endothelin 1 (ET-1)	100
ET-2	21
ET-3	3.6
Human Big ET-1	<0.1
Rat Big ET-1	<0.1
Human Big ET-2	<0.1
Human Big ET-3 Amide	<0.1

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 20, "mean": "1.2 pg/mL", "standardDeviation": null, "coefficientOfVariability": "15.3" }, { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 20, "mean": "2.5 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 20, "mean": "35.1 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 20, "mean": "35.9 pg/mL", "standardDeviation": null, "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 20, "mean": "1.1 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 20, "mean": "2.3 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8.9" } ]

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Endothelin 1 (ET-1) is a potent vasoconstrictor peptide with a molecular mass of approximately 2.5 kDa. The gene encoding ET-1 is expressed predominantly in endothelial cells where it exerts its vasoconstrictive effect. It is synthesized as a prepropeptide that undergoes cleavage to form the mature ET-1 peptide. The primary expression occurs in the vascular endothelial cells but it is also found in the kidney lung and brain indicating its widespread physiological role.

Biological function summary

ET-1 regulates vascular tonus and blood pressure. It is part of the endothelin family that includes endothelin-2 (ET-2) and endothelin-3 (ET-3). ET-1 binds to endothelin receptors such as ETA and ETB which are G protein-coupled receptors facilitating cellular signaling processes. These receptors are abundant in smooth muscle cells and the central nervous system. The interaction of ET-1 with these receptors affects not only vascular smooth muscle contraction but also cell proliferation and production of extracellular matrix proteins.

Pathways

ET-1 plays an important role in the renin-angiotensin-aldosterone system and the nitric oxide signaling pathway. ET-1 interacts closely with angiotensin II a peptide hormone that increases blood pressure by stimulating the production of endothelin in endothelial cells. The release of nitric oxide by endothelium serves as a counter-regulatory mechanism to ET-1-mediated vasoconstriction. The balance between ET-1 and nitric oxide is vital for maintaining vascular homeostasis.

ET-1 is significantly implicated in hypertension and pulmonary arterial hypertension (PAH). The overproduction of ET-1 contributes to sustained high blood pressure and arterial stiffness. In PAH ET-1 levels are increased leading to the constriction and proliferation of pulmonary vascular cells. ET-1's interaction with proteins like transforming growth factor-beta (TGF-β) further enhances its role in vascular remodeling associated with these conditions. Understanding ET-1's interaction in these pathways has made it a therapeutic target for treating these cardiovascular diseases.

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产品实验方案

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靶点信息

Endothelins are endothelium-derived vasoconstrictor peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (PubMed : 19086031). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437). Promotes mesenteric arterial wall remodeling via activation of ROCK signaling and subsequent colocalization of NFATC3 with F-actin filaments (By similarity). NFATC3 then translocates to the nucleus where it subsequently promotes the transcription of the smooth muscle hypertrophy and differentiation marker ACTA2 (By similarity).

See full target information EDN1

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文献 (31)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23092 PubMed37482902

2023

Blockade of the mineralocorticoid receptor improves markers of human endothelial cell dysfunction and hematological indices in a mouse model of sickle cell disease.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia Rivera,Christopher Vega,Arelys Ramos-Rivera,Enrique R Maldonado,Gregory N Prado,Hope E Karnes,Yuri A Fesko,L Michael Snyder,Seth L Alper,Jose R Romero

International journal of molecular sciences 24: PubMed37108136

2023

Dual Blockade of TGF-β Receptor and Endothelin Receptor Synergistically Inhibits Angiotensin II-Induced Myofibroblast Differentiation: Role of ATR/G-Mediated TGF-β1 and ET-1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ratchanee Duangrat,Warisara Parichatikanond,Supachoke Mangmool

BMC endocrine disorders 22:293 PubMed36434695

2022

Novel sedentary cage induced sedentariness in rats: evidence from relevant biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Quadri K Alabi,Rufus O Akomolafe

Diabetes & vascular disease research 19:14791641221131788 PubMed36357361

2022

Monitoring and recovery of hyperglycaemia-induced endothelial dysfunction with rheopheresis in diabetic lower extremity ulceration with hyperviscosity.

Applications

Unspecified application

Species

Unspecified reactive species

Kristóf Gál,Georgina Asbóth,Melinda Vass,Attila Bíró,Arnold Markovich,Judit Homoki,Gábor Fidler,Melinda Paholcsek,Zoltán Cziáky,Norbert Németh,Judit Remenyik,Pál Soltész

Frontiers in cardiovascular medicine 9:1025558 PubMed36426231

2022

Repair effect of the poly (D,L-lactic acid) nanoparticle containing tauroursodeoxycholic acid-eluting stents on endothelial injury after stent implantation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiedong Zhou,Jingfan Weng,Xingxiao Huang,Shimin Sun,Qi Yang,Hui Lin,Jinjin Yang,Hangyuan Guo,Jufang Chi

Frontiers in neurology 13:935579 PubMed35959408

2022

"Rogue" [DEspR+CD11b+] neutrophil subset correlates with severity in spontaneous intracerebral hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria L M Herrera,Courtney E Takahashi,Mai Q Nguyen,Julie Z Mosaddeghi,Ridiane Denis,David M Greer,Nelson Ruiz-Opazo

Frontiers in cardiovascular medicine 9:695004 PubMed35865382

2022

Cardioprotective Effect of Paeonol on Chronic Heart Failure Induced by Doxorubicin Regulating the miR-21-5p/S-Phase Kinase-Associated Protein 2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Chen,Shuhong Liu,Gaozhen Cao,Yang Hu,Run Wang,Min Wu,Mingya Liu,Kai Hang Yiu

International journal of molecular sciences 23: PubMed35457157

2022

Aortic Fibrosis in Insulin-Sensitive Mice with Endothelial Cell-Specific Deletion of Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Raghd Abu Helal,Harrison T Muturi,Abraham D Lee,Wei Li,Hilda E Ghadieh,Sonia M Najjar

Scientific reports 12:5583 PubMed35379853

2022

A targetable 'rogue' neutrophil-subset, [CD11b+DEspR+] immunotype, is associated with severity and mortality in acute respiratory distress syndrome (ARDS) and COVID-19-ARDS.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria L M Herrera,Allan J Walkey,Mai Q Nguyen,Christopher M Gromisch,Julie Z Mosaddhegi,Matthew S Gromisch,Bakr Jundi,Soeren Lukassen,Saskia Carstensen,Ridiane Denis,Anna C Belkina,Rebecca M Baron,Mayra Pinilla-Vera,Meike Mueller,W Taylor Kimberly,Joshua N Goldstein,Irina Lehmann,Angela R Shih,Roland Eils,Bruce D Levy,Nelson Ruiz-Opazo

Inflammation 45:1651-1667 PubMed35199285

2022

The Prospective Effect of Allopurinol on the Oxidative Stress Index and Endothelial Dysfunction in Covid-19.

Applications

Unspecified application

Species

Unspecified reactive species

Hayder M Al-Kuraishy,Ali I Al-Gareeb,Marwa S Al-Niemi,Reem M Aljowaie,Saeedah Musaed Almutairi,Athanasios Alexiou,Gaber El-Saber Batiha

View all publications

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websiteProtocolBooklet

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Endothelin 1 ELISA试剂盒

Endothelin 1 ELISA Kit

Sandwich ELISA - Endothelin 1 ELISA Kit (AB133030)

Sandwich ELISA - Endothelin 1 ELISA Kit (AB133030)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台