JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283541

Dystrophin ELISA试剂盒

Dystrophin ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Dystrophin ELISA Kit is a single-wash 90-min Simplestep used to quantify Dystrophin with a sensitivity of 15.12 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Dystrophin, DMD

3 Images
ELISA - Dystrophin ELISA Kit (AB283541)
  • ELISA

Supplier Data

ELISA - Dystrophin ELISA Kit (AB283541)
Sandwich ELISA - Dystrophin ELISA Kit (AB283541)
  • sELISA

Supplier Data

Sandwich ELISA - Dystrophin ELISA Kit (AB283541)

Interpolated concentrations of native Dystrophin in human HepG2 cell extract and mouse heart homogenate extract, based on 250 μg/mL and 500 μg/mL extract loads, respectively.

The concentrations of Dystrophin were measured in duplicate and interpolated from the Dystrophin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Dystrophin concentration was determined to be 2,365.64 pg/mL in HepG2 extract and 1,273.63 pg/mL in mouse heart homogenate extract.

Sandwich ELISA - Dystrophin ELISA Kit (AB283541)
  • sELISA

Supplier Data

Sandwich ELISA - Dystrophin ELISA Kit (AB283541)

Example of human Dystrophin standard curve in 1X Cell Extraction Buffer PTR.

The Dystrophin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Tissue, Cell Lysate

反应种属

Human, Mouse

检测类型

Sandwich

灵敏度

= 15.12 pg/mL

范围

117.188 - 7500 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Dystrophin ELISA Kit ab283541 is a rapid single-wash 90-min Sandwich ELISA to measure Dystrophin in cell lysate, tissue. This SimpleStep sensitivity is 15.12 pg/mL.

How the assay works

Dystrophin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Dystrophin ELISA Kit ab283541 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Dystrophin is a cytoskeletal protein that is a part of the dystrophin-associated glycoprotein complex that forms at the neuromuscular junction and in synapses in the nervous system. This complex connects the cytoskeleton of muscle fiber to the extracellular matrix. Mutations in dystrophin cause muscular dystrophy, a disease that leads to progressive muscle wasting.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
style
left-column

回收率

[ { "sample": "Cell Lysate", "range": null, "average": "= 118" }, { "sample": "Tissue Extracts", "range": null, "average": "= 112" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283541-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283541-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB283541-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB283541-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Dystrophin Lyophilized Recombinant Protein", "number":"AB283541-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB283541-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Dystrophin Capture Antibody", "number":"AB283541-CMP01", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB283541-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB283541-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Dystrophin Detector Antibody", "number":"AB283541-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB283541-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB283541-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB283541-CMP11", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Dystrophin also known as the DMD protein plays a mechanical role in muscle fibers by connecting the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. This structural connection helps reinforce the muscle fiber during contraction and mechanical stress. The protein has a molecular weight of approximately 427 kDa. It is expressed mainly in skeletal and cardiac muscles where it is important for maintaining muscle integrity.
Biological function summary

Dystrophin acts as an important component of the dystrophin-glycoprotein complex. This complex stabilizes the muscle cell membrane by linking actin filaments within the cytoskeleton to proteins in the extracellular matrix. The absence or malfunctioning of dystrophin disrupts this connection leading to increased susceptibility to damage during muscle contraction. This is especially evident in tissues where the protein is abundantly present.

Pathways

Dystrophin is integral to the structural integrity pathway in muscle cells. It works alongside proteins like dystroglycan and sarcoglycan forming a multiprotein complex that ensures cell membrane stability during muscle contractions. The proper functioning of the dystrophin complex is also linked to calcium signaling pathways highlighting its role in cellular signaling mechanisms.

Dystrophin's malfunction is directly associated with Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). Mutations in the DMD gene which encodes the dystrophin protein result in the absence or reduced functionality of the protein leading to progressive muscle degeneration observed in DMD and BMD. These disorders frequently involve the protein utrophin which sometimes compensates for the lack of functional dystrophin albeit insufficiently to alleviate the symptoms.
style
left-column

产品实验方案

style
left-column

靶点信息

Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.
See full target information DMD
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

搭配使用产品

Assay Kits

AB200016

Human Cardiac Troponin I ELISA Kit

Recombinant|SimpleStep

ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)

  • 5
  • 2
Primary Antibodies

AB15277

Anti-Dystrophin antibody

20ul selling size

Immunohistochemistry - Anti-Dystrophin antibody (AB15277)

  • 4
  • 23
Assay Kits

AB174447

Human Clusterin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Clusterin ELISA Kit (AB174447)

  • 0
  • 0
Primary Antibodies

AB32577

Anti-Caveolin-1 antibody [E249] - Caveolae Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (AB32577)

  • 5
  • 2

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com