Anti-Coxiella burnetii Phase 2 IgM ELISA试剂盒(Q-Fever) (ab178640)
Key features and details
- Sensitivity: 90 %
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
概述
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产品名称
Anti-Coxiella burnetii Phase 2 IgM ELISA试剂盒(Q-Fever) -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Pos serum 15 2.9% Pos serum 15 2% 批次间 样品 n Mean SD CV% Pos serum 6 7.7% Pos serum 6 2.4% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Indirect -
灵敏度
> 90 % -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s anti-Coxiella burnetii (Q-Fever) Phase 2 IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Coxiella burnetii in Human serum.
A 96-well plate has been precoated with Coxiella burnetii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Coxiella burnetii antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Coxiella burnetii IgG sample captured in plate.
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经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit Coxiella burnetii Anti-IgM HRP Conjugate 1 x 20ml Coxiella burnetii IgM Cut-off Control 1 x 3ml Coxiella burnetii IgM Negative Control 1 x 2ml Coxiella burnetii IgM Positive Control 1 x 2ml Coxiella burnetii Phase 2 (IgM) Coated Microplate (12 x 8 wells) 1 unit IgM Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究领域
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相关性
Coxiella burnetii is an obligate intracellular bacterial pathogen, and is the causative agent of Q fever. The genus Coxiella is morphologically similar to Rickettsia, but with a variety of genetic and physiological differences. C. burnetii is a small Gram-negative bacterium that is highly resistant to environmental stresses such as high temperature, osmotic pressure, and ultraviolet light. These characteristics are attributed to a small cell variant (SCV) form of the organism that is part of a biphasic developmental cycle, including a more metabolically and replicatively active large cell variant (LCV) form. It can survive standard disinfectants, and is resistant to many other environmental changes like those presented in the phagolysosome.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178640于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab178640 被引用在 1 文献中.
- Tagoe JNA et al. Coinfection of Malaria and Bacterial Pathogens among Acute Febrile Patients in Selected Clinics in Ghana. Am J Trop Med Hyg 109:1036-1046 (2023). PubMed: 37748764