COVID-19 N-Protein人IgG ELISA试剂盒(ab274339)
Key features and details
- Range: 1.37 U/ml - 1000 U/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Quantitative
概述
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产品名称
COVID-19 N-Protein人IgG ELISA试剂盒
参阅全部 COVID-19 N-Protein 试剂盒 -
检测方法
Colorimetric -
样品类型
Serum -
检测类型
Quantitative -
范围
1.37 U/ml - 1000 U/ml -
实验步骤
Multiple steps standard assay -
产品概述
COVID-19 N-Protein Human IgG ELISA Kit (ab274339) is an in vitro indirect ELISA for the quantitative measurement of human IgG antibody against SARS-CoV-2 N protein in human serum.
Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARS-CoV-2 N protein, which combines with the corresponding antibody present in a sample and Positive Control which used as calibration curve for interpretation purposes. The wells are washed, and biotinylated anti-human IgG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID19 N protein human IgG antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Controls are from an inactivated serum sample which contains SARS-COV-2 N protein human IgG antibody. We do not know the exact amount of SARS-COV-2 N protein human IgG antibody in the Positive Control sample. The Positive Control can be used as calibration curve for interpretation purposes in different assays.
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -80°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Sample Diluent 1 x 25ml Biotinylated anti-Human IgG Antibody 1 vial HRP-Streptavidin Concentrate 1 vial Positive Control 1 vial SARS-CoV-2 N protein coated Microplate 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab274339于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
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Example data
Example Positive Control calibration curve.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab274339 被引用在 3 文献中.
- Bueno-Hernández N et al. High Incidence Rate of SARS-CoV-2 Infection in Health Care Workers at a Dedicated COVID-19 Hospital: Experiences of the Pandemic from a Large Mexican Hospital. Healthcare (Basel) 10:N/A (2022). PubMed: 35628032
- Viurcos-Sanabria R et al. In Vitro Exposure of Primary Human T Cells and Monocytes to Polyclonal Stimuli Reveals a Basal Susceptibility to Display an Impaired Cellular Immune Response and Develop Severe COVID-19. Front Immunol 13:897995 (2022). PubMed: 35860236
- McCarthy P et al. A Novel Dry-Stabilized Whole Blood Microsampling and Protein Extraction Method for Testing of SARS-CoV-2 Antibody Titers. Vaccines (Basel) 10:N/A (2022). PubMed: 36298625