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AB274339

COVID-19 N-Protein人IgG ELISA试剂盒

COVID-19 N-Protein Human IgG ELISA Kit

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(5 Publications)

COVID-19 N-Protein Human IgG ELISA Kit is a ELISA for the measurement of COVID-19 N-Protein Human IgG in in Biofluids samples.
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ELISA - COVID-19 N-Protein Human IgG ELISA Kit (AB274339)
  • ELISA

Supplier Data

ELISA - COVID-19 N-Protein Human IgG ELISA Kit (AB274339)

Example Positive Control calibration curve.

关键信息

检测方法

Colorimetric

样品类型

Serum

范围

1.37 - 1000 U/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

COVID-19 N-Protein Human IgG ELISA Kit (ab274339) is an in vitro indirect ELISA for the quantitative measurement of human IgG antibody against SARS-CoV-2 N protein in human serum.

Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARS-CoV-2 N protein, which combines with the corresponding antibody present in a sample and Positive Control which used as calibration curve for interpretation purposes. The wells are washed, and biotinylated anti-human IgG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID19 N protein human IgG antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

The Positive Controls are from an inactivated serum sample which contains SARS-COV-2 N protein human IgG antibody. We do not know the exact amount of SARS-COV-2 N protein human IgG antibody in the Positive Control sample. The Positive Control can be used as calibration curve for interpretation purposes in different assays.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab274339-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB274339-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB274339-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Biotinylated anti-Human IgG Antibody", "number":"AB274339-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SARS-CoV-2 N protein coated Microplate", "number":"AB274339-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB274339-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"5X Sample Diluent", "number":"AB274339-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"HRP-Streptavidin Concentrate", "number":"AB274339-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control", "number":"AB274339-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB274339-CMP05", "productcode":"" } ] } } }

性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
-80°C

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

COVID-19 N-Protein also known as the nucleocapsid protein of SARS-CoV-2 plays a fundamental role in the virus's life cycle. Mechanically it binds to the viral RNA genome forming a ribonucleoprotein complex that is critical for viral replication. N-Protein's approximate molecular mass is around 46 kDa. It is expressed in the cytoplasm of infected cells and is highly abundant making it an important target for diagnostic assays like the COVID-19 ELISA test.
Biological function summary

The N-Protein functions as a structural component of the coronavirus. It facilitates the encapsidation of the viral genome and forms part of the viral replication-transcription complexes. The N-Protein interacts with other viral proteins such as the membrane (M) protein indicating its involvement in viral assembly and release. It influences the host cell's response to infection by modulating various cellular processes.

Pathways

The COVID-19 N-Protein participates in the replication and transcription pathway of SARS-CoV-2. It engages with RNA polymerase and NSP3 to support efficient viral RNA synthesis. This protein is related to the structural pathway of viral particles where it ensures the stability and packaging of the viral genome alongside proteins like the S (spike) and E (envelope) proteins.

The COVID-19 N-Protein is closely associated with the pathogenesis of COVID-19. It triggers an immune response making it a target for antibody detection in serological assays such as the COVID-19 ELISA kit. The N-Protein's interaction with host cell components may influence disease severity by affecting interferon pathways linked to other structural proteins like the S-protein. Understanding these interactions helps in developing therapeutic strategies against COVID-19.

产品实验方案

靶点信息

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0325379 PubMed40472047

2025

Long-term pulmonary sequelae and convalescent immune reactions in mild to moderate COVID-19 patients during the active treatment era.

Applications

Unspecified application

Species

Unspecified reactive species

Minkyeong Lee,Byoung Kwon Park,Dong Hoon Shin,Hong Sang Oh,Chae-Hong Jeong,So-Young Lee,Jungyeon Kim,Sang-Won Park

PloS one 19:e0297901 PubMed38416704

2024

Risk factors contributing to infection with SARS-CoV-2 are modulated by sex.

Applications

Unspecified application

Species

Unspecified reactive species

Octavio A Lecona,América G Arroyo-Valerio,Nallely Bueno-Hernández,José Damian Carrillo-Ruíz,Luis Ruelas,René Márquez-Franco,Alejandro Aguado-García,Eira Valeria Barrón,Galileo Escobedo,Elizabeth Ibarra-Coronado,Paola V Olguín-Rodríguez,Antonio Barajas-Martínez,Ana Leonor Rivera,Ruben Fossion

Vaccines 10: PubMed36298625

2022

A Novel Dry-Stabilized Whole Blood Microsampling and Protein Extraction Method for Testing of SARS-CoV-2 Antibody Titers.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick McCarthy,Joseph A Pathakamuri,Daniel Kuebler,Jocelyn Neves,Madison Krohn,Michael Rohall,Isaac Archibeque,Heidi Giese,Martina Werner,Eugenio Daviso,Ulrich Thomann

Frontiers in immunology 13:897995 PubMed35860236

2022

Exposure of Primary Human T Cells and Monocytes to Polyclonal Stimuli Reveals a Basal Susceptibility to Display an Impaired Cellular Immune Response and Develop Severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Rebeca Viurcos-Sanabria,Aarón N Manjarrez-Reyna,Helena Solleiro-Villavicencio,Salma A Rizo-Téllez,Lucía A Méndez-García,Victoria Viurcos-Sanabria,Jacquelina González-Sanabria,América Arroyo-Valerio,José D Carrillo-Ruíz,Antonio González-Chávez,Jose I León-Pedroza,Raúl Flores-Mejía,Octavio Rodríguez-Cortés,Galileo Escobedo

Healthcare (Basel, Switzerland) 10: PubMed35628032

2022

High Incidence Rate of SARS-CoV-2 Infection in Health Care Workers at a Dedicated COVID-19 Hospital: Experiences of the Pandemic from a Large Mexican Hospital.

Applications

Unspecified application

Species

Unspecified reactive species

Nallely Bueno-Hernández,José Damian Carrillo-Ruíz,Lucía A Méndez-García,Salma A Rizo-Téllez,Rebeca Viurcos-Sanabria,Alisson Santoyo-Chávez,René Márquez-Franco,Alejandro Aguado-García,Neyla Baltazar-López,Yoshio Tomita-Cruz,Eira Valeria Barrón,Ana Laura Sánchez,Edna Márquez,Ruben Fossion,Ana Leonor Rivera,Luis Ruelas,Octavio A Lecona,Gustavo Martínez-Mekler,Markus Müller,América G Arroyo-Valerio,Galileo Escobedo
View all publications
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