AB276183

COVID-19 N-Protein人IgA ELISA试剂盒

Name: COVID-19 N-Protein 人IgA ELISA试剂盒 (ab276183)| Abcam中文官网
Brand: Abcam Limited
SKU: AB276183
Price: 11854 CNY
Availability: InStock

COVID-19 N-Protein Human IgA ELISA Kit

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(1 Publication)

COVID-19 N-Protein Human IgA ELISA Kit is a ELISA for the measurement of COVID-19 N-Protein Human IgA in in Biofluids samples.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Serum

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

COVID-19 N-Protein Human IgA ELISA Kit (ab276183) is an in vitro indirect ELISA for the semi-quantitative measurement of human IgA antibody against SARS-CoV-2 N protein in human serum.

Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARSCoV-2 N protein, which combines with the corresponding antibody present in a sample. When a secondary anti-human Antibody-HRP is added, a complex of Antibody-HRP human IgA antibody virus N antigen forms on the microplate. A TMB substrate is added and a blue color is generated. The depth of color is relative to the amount of the anti-SARS-CoV-2 IgA antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

The Positive Control is from an inactivated serum sample which contains SARS-COV-2 N protein human IgA antibody. We do not know the exact amount of SARS-COV-2 N protein human IgA antibody in the Positive Control sample. The Positive Control can be used as calibration curve for interpretation purposes in different assays.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab276183-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB276183-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB276183-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SARS-CoV-2 N protein coated Microplate", "number":"AB276183-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control", "number":"AB276183-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"HRP-anti IgA antibody", "number":"AB276183-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB276183-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB276183-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Negative Control", "number":"AB276183-CMP07", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Dry Ice

储存信息

-80°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

COVID-19 N-Protein also known as the nucleocapsid protein of SARS-CoV-2 plays a fundamental role in the virus's life cycle. Mechanically it binds to the viral RNA genome forming a ribonucleoprotein complex that is critical for viral replication. N-Protein's approximate molecular mass is around 46 kDa. It is expressed in the cytoplasm of infected cells and is highly abundant making it an important target for diagnostic assays like the COVID-19 ELISA test.

Biological function summary

The N-Protein functions as a structural component of the coronavirus. It facilitates the encapsidation of the viral genome and forms part of the viral replication-transcription complexes. The N-Protein interacts with other viral proteins such as the membrane (M) protein indicating its involvement in viral assembly and release. It influences the host cell's response to infection by modulating various cellular processes.

Pathways

The COVID-19 N-Protein participates in the replication and transcription pathway of SARS-CoV-2. It engages with RNA polymerase and NSP3 to support efficient viral RNA synthesis. This protein is related to the structural pathway of viral particles where it ensures the stability and packaging of the viral genome alongside proteins like the S (spike) and E (envelope) proteins.

The COVID-19 N-Protein is closely associated with the pathogenesis of COVID-19. It triggers an immune response making it a target for antibody detection in serological assays such as the COVID-19 ELISA kit. The N-Protein's interaction with host cell components may influence disease severity by affecting interferon pathways linked to other structural proteins like the S-protein. Understanding these interactions helps in developing therapeutic strategies against COVID-19.

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产品实验方案

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靶点信息

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:832924 PubMed35935974

2022

Local and Systemic Immunity Are Impaired in End-Stage-Renal-Disease Patients Treated With Hemodialysis, Peritoneal Dialysis and Kidney Transplant Recipients Immunized With BNT162b2 Pfizer-BioNTech SARS-CoV-2 Vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Piotrowska,Maciej Zieliński,Leszek Tylicki,Bogdan Biedunkiewicz,Alicja Kubanek,Zuzanna Ślizień,Karolina Polewska,Piotr Tylicki,Marta Muchlado,Justyna Sakowska,Marcin Renke,Adam Sudoł,Małgorzata Dąbrowska,Monika Lichodziejewska-Niemierko,Tomasz Smiatacz,Alicja Dębska-Ślizień,Piotr Trzonkowski

View all publications

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websiteProtocolBooklet

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COVID-19 N-Protein人IgA ELISA试剂盒

COVID-19 N-Protein Human IgA ELISA Kit