COVID-19 N-Protein ELISA试剂盒(ab274341)
Key features and details
- Sensitivity: 0.07 ng/ml
- Range: 0.069 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
概述
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产品名称
COVID-19 N-Protein ELISA试剂盒
参阅全部 COVID-19 N-Protein 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum -
检测类型
Quantitative -
灵敏度
0.07 ng/ml -
范围
0.069 ng/ml - 50 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 97.9 83% - 111% Cell culture media 87.26 83% - 92% -
实验步骤
Multiple steps standard assay -
产品概述
COVID-19 N-Protein ELISA Kit (ab274341) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of COVID-19 N Protein in serum (plasma is not recommended in this assay) and cell culture supernatants.
This assay employs an antibody specific for COVID-19 N Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and COVID-19 N Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-COVID-19 N Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID-19 N Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Antibody 2 vials 100X HRP-Strepavidin Concentrate 1 x 200µl Lyophilized Standard Protein 2 vials Microplate 1 unit Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab274341于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
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COVID-19 N Protein Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab274341 被引用在 1 文献中.
- Valiente E et al. Correlation of SARS-CoV-2 RNA and nucleocapsid concentrations in samples used in INSTAND external quality assessment schemes. BMC Res Notes 16:210 (2023). PubMed: 37697348