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AB274341

COVID-19 N-Protein ELISA试剂盒

COVID-19 N-Protein ELISA Kit

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(1 Publication)

COVID-19 N-Protein ELISA Kit is a ELISA for the measurement of COVID-19 N-Protein in in Cell Culture Media, Biofluids samples.

查看别名

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

1 Images
Functional Studies - COVID-19 N-Protein ELISA Kit (AB274341)
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Supplier Data

Functional Studies - COVID-19 N-Protein ELISA Kit (AB274341)

COVID-19 N Protein Standard Curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Cell culture supernatant, Serum

灵敏度

= 0.07 ng/mL

范围

0.069 - 50 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

COVID-19 N-Protein ELISA Kit (ab274341) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of COVID-19 N Protein in serum (plasma is not recommended in this assay) and cell culture supernatants.

This assay employs an antibody specific for COVID-19 N Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and COVID-19 N Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-COVID-19 N Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID-19 N Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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回收率

[ { "sample": "Serum", "range": "83 - 111 %", "average": "= 97.9" }, { "sample": "Cell culture media", "range": "83 - 92 %", "average": "= 87.26" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab274341-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB274341-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Strepavidin Concentrate", "number":"AB274341-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate", "number":"AB274341-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB274341-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB274341-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard Protein", "number":"AB274341-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB274341-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Antibody", "number":"AB274341-CMP06", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

COVID-19 N-Protein also known as the nucleocapsid protein of SARS-CoV-2 plays a fundamental role in the virus's life cycle. Mechanically it binds to the viral RNA genome forming a ribonucleoprotein complex that is critical for viral replication. N-Protein's approximate molecular mass is around 46 kDa. It is expressed in the cytoplasm of infected cells and is highly abundant making it an important target for diagnostic assays like the COVID-19 ELISA test.
Biological function summary

The N-Protein functions as a structural component of the coronavirus. It facilitates the encapsidation of the viral genome and forms part of the viral replication-transcription complexes. The N-Protein interacts with other viral proteins such as the membrane (M) protein indicating its involvement in viral assembly and release. It influences the host cell's response to infection by modulating various cellular processes.

Pathways

The COVID-19 N-Protein participates in the replication and transcription pathway of SARS-CoV-2. It engages with RNA polymerase and NSP3 to support efficient viral RNA synthesis. This protein is related to the structural pathway of viral particles where it ensures the stability and packaging of the viral genome alongside proteins like the S (spike) and E (envelope) proteins.

The COVID-19 N-Protein is closely associated with the pathogenesis of COVID-19. It triggers an immune response making it a target for antibody detection in serological assays such as the COVID-19 ELISA kit. The N-Protein's interaction with host cell components may influence disease severity by affecting interferon pathways linked to other structural proteins like the S-protein. Understanding these interactions helps in developing therapeutic strategies against COVID-19.
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产品实验方案

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靶点信息

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury. Disrupts the association betwen NLRP3 and ABHD8, enhancing NLRP3 stability and leading to increased inflammasome activation (PubMed : 39225180).
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

BMC research notes 16:210 PubMed37697348

2023

Correlation of SARS-CoV-2 RNA and nucleocapsid concentrations in samples used in INSTAND external quality assessment schemes.

Applications

Unspecified application

Species

Unspecified reactive species

Esmeralda Valiente,Samreen Falak,Andreas Kummrow,Martin Kammel,Victor M Corman,Rainer Macdonald,Heinz Zeichhardt
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