Cortisol ELISA试剂盒(ab108674)
Key features and details
- Sensitivity: 2.95 ng/ml
- Range: 10 ng/ml - 500 ng/ml
- Sample type: Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
Cortisol ELISA试剂盒
参阅全部 Cortisol 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Urine 60 <=6.5% 批次间 样品 n Mean SD CV% Urine 30 <=7.2% -
样品类型
Urine -
检测类型
Competitive -
灵敏度
2.95 ng/ml -
范围
10 ng/ml - 500 ng/ml -
回收率
107.48 %
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实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in urine.
A 96-well plate has been precoated with anti-Cortisol. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Solution 1 x 50ml Anti-Cortisol IgG Coated Microplate (12 x 8 wells) 1 unit Cortisol Standard 0 - 0 ng/mL 1 x 4ml Cortisol Standard 1 - 1 ng/mL 1 x 1ml Cortisol Standard 2 - 5 ng/mL 1 x 1ml Cortisol Standard 3 - 30 ng/mL 1 x 1ml Cortisol Standard 4 – 200 ng/mL 1 x 1ml Cortisol-HRP Conjugate 1 x 33ml Cover foils 1 unit High control 1 x 1ml Low control 1 x 1ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108674于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108674
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108674 被引用在 1 文献中.
- Muhie S et al. Molecular alterations induced by Yersinia pestis, dengue virus and Staphylococcal enterotoxin B under severe stress. Brain Behav Immun 80:725-741 (2019). PubMed: 31100372