AB273258

鸡IL-6 ELISA试剂盒

Name: 鸡IL-6 ELISA试剂盒 (ab273258)| Abcam中文官网
Brand: Abcam Limited
SKU: AB273258
Price: 8173 CNY
Availability: InStock

Chicken IL-6 ELISA Kit

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(1 Publication)

Chicken IL-6 ELISA Kit is a Sandwich ELISA for the measurement of Chicken IL-6 in Chicken in Cell Culture Media, Biofluids samples.

查看别名

Interleukin-6, ChIL-6, IL-6, IL6

1 Images

Sandwich ELISA - Chicken IL-6 ELISA Kit (AB273258)

sELISA

Supplier Data

Sandwich ELISA - Chicken IL-6 ELISA Kit (AB273258)

Chicken IL-6 ELISA Kit standard curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Chicken IL-6 ELISA kit (ab273258) Standard curve.

关键信息

检测方法

Colorimetric

样品类型

Cell culture supernatant, Serum, EDTA Plasma

反应种属

Chicken

检测类型

Sandwich

灵敏度

= 0.085 ng/mL

范围

0.082 - 20 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Chicken IL-6 ELISA Kit (ab273258) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IL-6 in serum, EDTA plasma (Citrate and heparin have not been validated yet) and cell culture supernatants.

This assay employs an antibody specific for Chicken IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]

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回收率

[ { "sample": "Serum", "range": "71 - 114 %", "average": "= 96.57" }, { "sample": "Cell culture media", "range": "116 - 140 %", "average": "= 129.7" }, { "sample": "Plasma", "range": "72 - 80 %", "average": "= 76.16" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273258-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273258-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Chicken IL-6 Antibody", "number":"AB273258-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273258-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273258-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273258-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273258-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Chicken IL-6 coated Microplate (12 x 8 wells)", "number":"AB273258-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Chicken IL-6 Standard (Lyophilized)", "number":"AB273258-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB273258-CMP02", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.

Biological function summary

IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.

Pathways

IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.

IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.

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产品实验方案

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靶点信息

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6 : IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.

See full target information IL6

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of animal physiology and animal nutrition 106:132-138 PubMed33964044

2021

Tetramethylpyrazine supplementation improves performance, digestion, blood and immune state of broilers exposure to oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Hanyi Shi,Yiran Luo,Yufa Li,Feike Zhang,Ning Liu

View all publications

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websiteProtocolBooklet

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鸡IL-6 ELISA试剂盒

Chicken IL-6 ELISA Kit

Sandwich ELISA - Chicken IL-6 ELISA Kit (AB273258)