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AB277406

鸡IL-21 ELISA试剂盒

Chicken IL-21 ELISA Kit

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Chicken IL-21 ELISA Kit is a ELISA for the measurement of Chicken IL-21 in Chicken in Cell Culture Media, Biofluids samples.

查看别名

CVID11, IL21_HUMAN, Interleukin-21, OTTHUMP00000164088, Za11, interleukin-21 isoform

1 Images
ELISA - Chicken IL-21 ELISA Kit (AB277406)
  • ELISA

Supplier Data

ELISA - Chicken IL-21 ELISA Kit (AB277406)

These standard curves are for demonstration only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Cell culture supernatant, Serum, EDTA Plasma

反应种属

Chicken

灵敏度

= 4.5 pg/mL

范围

4.1 - 1000 pg/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Chicken IL-21 ELISA kit (ab277406) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IL-21 in serum (Chicken IL-21 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma EDTA (Citrate and Heparin have not been validated yet) and cell culture supernatants.

This assay employs an antibody specific for Chicken IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and Chicken IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Chicken IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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回收率

[ { "sample": "Serum", "range": "82 - 131 %", "average": "= 106.1" }, { "sample": "Cell culture media", "range": "83 - 124 %", "average": "= 102.7" }, { "sample": "Plasma", "range": "77 - 129 %", "average": "= 102.3" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277406-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277406-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277406-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277406-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Chicken IL-21 coated Microplate", "number":"AB277406-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Chicken IL-21 Antibody", "number":"AB277406-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277406-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB277406-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Chicken IL-21 standard protein (Lyophilized)", "number":"AB277406-CMP07", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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产品实验方案

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靶点信息

See full target information IL-21
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websiteProtocolBooklet
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