Canine Resistin ELISA试剂盒(ab273177)
Key features and details
- Sensitivity: 16 pg/ml
- Range: 16.38 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
概述
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产品名称
Canine Resistin ELISA试剂盒
参阅全部 Resistin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
16 pg/ml -
范围
16.38 pg/ml - 4000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 138.2 129% - 149% Plasma 118.4 85% - 150% Cell culture media 87.49 82% - 95% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Dog -
产品概述
Canine Resistin ELISA Kit (ab273177) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine Resistin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine Resistin coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine Resistin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine Resistin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine Resistin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Anti-Canine Resistin coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Canine Resistin Antibody 2 vials Canine Resistin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
组织特异性
Expressed only in fatty tissues. -
序列相似性
Belongs to the resistin/FIZZ family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273177于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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