Canine PDGF-BB ELISA试剂盒(ab273171)

Canine PDGF-BB ELISA试剂盒
参阅全部 PDGF B 试剂盒

Colorimetric

Cell culture supernatant, Serum, Plasma

Sandwich (quantitative)

4 pg/ml

12.29 pg/ml - 1200 pg/ml

特定样本回收率

样品类型	平均%	范围
Cell culture supernatant	141.6	134% - 148%
Serum	132.2	122% - 147%
Plasma	130.2	121% - 148%

Multiple steps standard assay

与反应: Dog

Canine PDGF-BB ELISA Kit (ab273171) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine PDGF-BB in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for canine PDGF-BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-PDGF-BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Pre-coated microplate (12 x 8 well strips)

Store at -20°C. Please refer to protocols.

• Cardiovascular
• Blood
• Serum Proteins

• Signal Transduction
• Growth Factors/Hormones
• PDGF

• Cancer
• Growth factors
• PDGF

• Cardiovascular
• Atherosclerosis
• Vascular Inflammation
• Inflammatory mediators

• Developmental Biology
• Organogenesis
• Angiogenesis and vasculogenesis

• Cancer
• Cancer Metabolism
• Response to hypoxia

• Metabolism
• Pathways and Processes
• Metabolism processes
• Hypoxia

• Metabolism
• Types of disease
• Heart disease