Canine IGFBP2 ELISA试剂盒(ab273184)
Key features and details
- Sensitivity: 0.055 ng/ml
- Range: 0.051 ng/ml - 12.5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
概述
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产品名称
Canine IGFBP2 ELISA试剂盒
参阅全部 IGFBP2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.055 ng/ml -
范围
0.051 ng/ml - 12.5 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 105.3 97% - 112% Plasma 98.77 91% - 104% Cell culture media 87.73 74% - 104% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Dog -
产品概述
Canine IGFBP2 ((Insulin-like Growth Factor Binding Protein 2) ELISA Kit (ab273184) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine IGFBP2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine IGFBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 550X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Canine IGFBP2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine IGFBP2 Antibody 2 vials Canine IGFBP2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
序列相似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
结构域
The C-terminus is required for IGF-binding and growth inhibition. -
翻译后修饰
O-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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别名
- BP 2
- BP2
- IBP 2
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273184于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Canine IGFBP2 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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