Canine HGF ELISA试剂盒(ab273174)
Key features and details
- Sensitivity: 0.24 ng/ml
- Range: 0.246 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
概述
-
产品名称
Canine HGF ELISA试剂盒
参阅全部 HGF 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.24 ng/ml -
范围
0.246 ng/ml - 60 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 94.38 79% - 116% Plasma 100.6 86% - 123% Cell culture media 126.5 110% - 145% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Dog -
产品概述
Canine HGF ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine HGF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Canine HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Canine HGF coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine HGF Antibody 2 vials Canine HGF Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
-
功能
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. -
疾病相关
Deafness, autosomal recessive, 39 -
序列相似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
-
别名
- DFNB39
- F TCF
- Fibroblast derived tumor cytotoxic factor
see all -
数据库链接
- Entrez Gene: 403441 Dog
- SwissProt: Q867B7 Dog
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273174于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
-
Canine HGF ELISA Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Canine HGF ELISA kit (ab273174) Standard curve.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab273174 尚未被引用在任何文献中。