Canine EPO ELISA试剂盒
Canine EPO ELISA kit
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Canine EPO ELISA kit is a Sandwich ELISA for the measurement of Canine EPO in Dog in Biofluids, Cell Culture Media samples.
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EP, EPO alpha, Epoetin, Erythropoetin, Erythropoietin precursor, MVCD2
- ELISA
Supplier Data
ELISA - Canine EPO ELISA kit (AB277400)
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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产品详情
Canine EPO ELISA Kit (ab277400) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine EPO in serum (Canine EPO concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Canine EPO coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine EPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine EPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine EPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Erythropoietin regulates blood oxygen levels by stimulating erythrocyte production. It is not part of a larger complex but interacts directly with the erythropoietin receptor activating intracellular pathways necessary for erythroid progenitor cell survival and proliferation. Eyrthropoietin production increases in response to low oxygen levels in tissues ensuring adequate oxygen delivery throughout the body by increasing red blood cell count.
Pathways
Erythropoietin predominantly influences the JAK-STAT signaling pathway. Upon binding to its receptor on erythroid progenitors it activates this pathway leading to gene expression changes that promote survival and proliferation of these cells. This pathway is also involved in various immune responses and cell growth processes. Erythropoietin signaling has a regulatory relationship with other growth factors like thrombopoietin which is another hematopoietic hormone.
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