Canine Cystatin C ELISA试剂盒(ab273173)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 10.24 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
概述
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产品名称
Canine Cystatin C ELISA试剂盒
参阅全部 Cystatin C 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
10 pg/ml -
范围
10.24 pg/ml - 2500 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 106.3 89% - 115% Serum 96.03 87% - 105% Plasma 97.36 79% - 112% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Dog
预测可用于: Mouse
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产品概述
Canine Cystatin C ELISA Kit (ab273173) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine Cystatin C in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine Cystatin C coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine Cystatin C present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine Cystatin C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine Cystatin C bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Canine Cystatin C coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine Cystatin C Antibody 2 vials Canine Cystatin C Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. -
组织特异性
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. -
疾病相关
Amyloidosis 6
Macular degeneration, age-related, 11 -
序列相似性
Belongs to the cystatin family. -
翻译后修饰
The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. -
细胞定位
Secreted. - Information by UniProt
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别名
- AD 8
- AD8
- Amyloid angiopathy and cerebral hemorrhage
see all -
数据库链接
- Entrez Gene: 13010 Mouse
- SwissProt: P21460 Mouse
- Unigene: 4263 Mouse
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273173于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Canine Cystatin C ELISA Kit standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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